Research Equipment: Pulsed Field Gradient NMR Diffusion Probe

9905569捆绑该项目支持为科罗拉多矿业学院(CSM)核磁共振设施的最先进的核磁共振(核磁共振)光谱仪采购脉冲场梯度(PFG)扩散探头。这项探索提供的实验能力将极大地增加CSM教职员工和学生的研究和教育机会。分布在四个不同部门的六个不同的研究项目最初将使用PFG探测器来产生重要的新科学和工程知识。此外，研究生和本科生将能够学习和使用这一现代工具，通过测量扩散和扩散的边界来研究复合介质和复杂材料。这种探头对于首席研究员对复合膜材料，特别是哺乳动物的表皮(皮肤)的研究将特别有价值。复合材料中的化学渗透可以通过一条或多条途径进行。对于复合材料，扩散池测量是量化通过膜的化学传输的传统方法，提供了关于整体传输的信息，但无法深入了解实际路径或这些路径中的局部扩散系数。使用PFG扩散探头，PI将测量选定的化学示踪剂(包括19F)在人类和动物受试者的角质层(SC)中的自扩散系数。SC是皮肤的最外层，也是大多数化学物质进出人体的主要屏障。这些扩散探针研究的目的是了解表皮屏障功能的机制。这一认识将被用于开发预测、增强和控制皮肤渗透性的方法，并用于开发替代皮肤样本以估计皮肤吸收的材料。用来研究SC复合膜的研究策略也将有益于其他复合膜的研究，无论是生物的还是非生物的。除了PI的项目外，CSM学院的其他五名成员(辅助用户)来自三个系(化学工程和石油精炼、工程和地球物理)，他们提出了利用PFG扩散探测器进行研究调查的建议。核磁共振-PFG方法已经在本提案中描述的所有研究项目中得到了演示，尽管测量的次数非常少，并且有很大的机会利用该设备产生新的知识。

9905569BungeThis project supports acquisition of a pulsed field gradient (PFG) diffusion probe for the state-of-the-art nuclear magnetic resonance (NMR) spectrometer at the Colorado School of Mines (CSM) NMR Facility. The experimental capabilities provided by this probe will greatly enhance research and educational opportunities to CSM's faculty and students. Six separate research programs housed in four different departments will initially use the PFG probe to produce significant new scientific and engineering knowledge. In addition, graduate and undergraduate students will be able to learn about and use this modern tool for investigating composite media and complex materials through measurements of diffusion and boundaries to diffusion. This probe will be particularly valuable to the principal investigator's (PI's) studies of composite membrane materials, specifically mammalian epidermis (skin). Chemical penetration in composite materials can proceed by one or more pathways. For composite materials, diffusion cell measurements, the traditional method of quantifying chemical transport through membranes, provide information on overall transport, but no insights into the actual pathways or the local diffusion coefficients in those paths. Using the PFG diffusion probe, the PI will measure self-diffusion coefficients of selected chemical tracers (those that include 19F) in stratum corneum (SC) excised from human and animal subjects. The SC is the outermost layer of skin and the main barrier to passage of most chemicals into and out of the body. The aim of these diffusion probe studies is to understand the mechanisms of epidermal barrier function. This understanding will then be used in developing methods to predict, enhance, and control dermal penetration and in developing materials to replace skin samples for estimating dermal absorption. The investigative strategies used to study the SC composite membrane will also be useful in the study of other complex membranes, either biological or non-biological. In addition to the PI's program, five other members of the CSM faculty (auxiliary users) from three departments (Chemical Engineering and Petroleum Refining, Engineering, and Geophysics) have proposed research investigations utilizing the PFG diffusion probe. The NMR-PFG method has been demonstrated for all of the research projects described in this proposal, although the number of measurements is extremely small and there is a great opportunity to produce new knowledge with this device.