Dissertation Enhancement: Immunolocalization of a Dehydrin-like Protein in Cornus sericea Xylem

9908427 Ashtek对该项目的支持将使博士Dale Karlson先生能够2005年，我与普渡大学园艺与景观建筑系Edward Ashworth博士合作，在日本京都大学木材研究所Takao Itoh博士的实验室进行论文研究。 研究人员将致力于了解植物极端耐寒性的分子方面。 该项目的目的是利用免疫定位技术来表征24 kDa的类胰蛋白酶木质部蛋白的位置，以确定其在冷驯化过程中增强红柳山茱萸（C.绢毛苔属）。 低温胁迫是植物分布和生产力的主要限制因素。 C.绢蒿属植物是世界上最耐寒的植物之一。 关于冷驯化相关蛋白在该物种中积累的调查可能会提供对极端耐受生物体特有的特定分子特征的了解。 这样的见解将增加对耐冻性这一复杂性状的理解，对农业具有重要意义。 该项目将受益于两个实验室的优势互补。 特别是，研究生将受益于伊藤博士在木本植物组织超微切片方面的独特专业知识。

9908427AshworthSupport for this project will enable Mr. Dale Karlson, a Ph.D. candidate with Dr. Edward Ashworth, Department of Horticulture and Landscape Architecture, Purdue University, to conduct dissertation research in the laboratory of Dr. Takao Itoh, Wood Research Institute, Kyoto University, Japan. The researchers will work to understand the molecular aspects of extreme cold hardiness in plants. The objective of the project is to characterize the location of a 24 kDa dehydrin-like xylem protein, using immunolocalization, to ascertain its potential functional roles for enhancement of freezing tolerance during cold acclimation in the red osier dogwood (C. sericea). Survival of low temperature stress is a major limiting factor of plant distribution and productivity. C. Sericea is one of the most freeze tolerant plant species. Investigations concerning the accumulation of cold acclimation related proteins in this species would potentially provide insight on specific molecular characteristics unique to extremely tolerant organisms. Such insight would increase understanding of the complex trait of freeze tolerance, with important implications for agriculture. This project will benefit from the complementary strengths of the two laboratories. In particular, the graduate student will benefit from Dr. Itoh's unique expertise in ultra-microtomy of woody plant tissue.