Developing Research-based Laboratories in Cell Biology Using Green Fluorescent Protein as a Unifying Theme

以绿色荧光蛋白为统一主题，发展细胞生物学研究型实验室

• 批准号: 9952672
• 负责人: Dunne Fong
• 金额: $ 8.8万
• 依托单位: Rutgers University New Brunswick
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-01 至 2003-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9952672&HistoricalAwards=false
• 关键词: Developing; Research; based; Laboratories; Cell

Biological Science (61) This new laboratory course in cell biology, part of the recently approved major in Cell Biology and Neuroscience, consists of three inter-related modules. The modules are designed to develop essential laboratory skills in cell biology, as well as more general skills emphasized in both the Rutgers University learning goals and the new curriculum, including critical thinking and problem solving. Participants acquire key skills in cell biology, including bacterial and mammalian cell culture, organelle isolation, recombinant DNA methodology and fluorescence microscopy. Green fluorescent protein (GFP), the basic research material of the modules, provides a unifying theme throughout the course. After an introduction to cutting-edge methods in cell biology, undergraduate students undertake a guided research project. They are prepared for this endeavor by reading assignments, discussion of research papers, and group meetings with guest faculties, who bring individual insights in their areas of expertise. From this experience, the students design and then complete the guided project, utilizing GFP as a research tool. Final reports are delivered orally, written up as if for publication, and revised versions published on the Internet. By participating in hypothesis-driven and inquiry-based laboratory projects, with communication skills linked to the course projects, students increase their ability to think logically, write clearly, and speak coherently. The project adapts and implements, and is based on, a number of studies showing the need for and effective incorporation of research into undergraduate courses (Brown, 1988 and Kenny, 1998). The modules described adapt and implement techniques outlined in research articles citing the ease of use of Green Fluorescent Protein as a marker and research tool (Misteli, 1997 and Conn, 1999).

生物科学(61)这门新的细胞生物学实验室课程是最近批准的细胞生物学和神经科学专业的一部分，由三个相互关联的模块组成。这些模块旨在培养细胞生物学的基本实验室技能，以及罗格斯大学的学习目标和新课程中强调的更一般的技能，包括批判性思维和解决问题。学员掌握细胞生物学的关键技能，包括细菌和哺乳动物细胞培养、细胞器分离、重组DNA方法学和荧光显微镜。绿色荧光蛋白(GFP)是这些模块的基础研究材料，在整个课程中提供了一个统一的主题。在介绍了细胞生物学的尖端方法之后，本科生将进行一项有指导意义的研究项目。他们通过阅读作业、研究论文的讨论和与客座学院的小组会议为这项工作做好准备，客座学院在他们的专业领域带来了个人的见解。根据这一经验，学生利用GFP作为研究工具，设计并完成指导项目。最终报告以口头形式提交，以书面形式发布，并在互联网上发布修订版本。通过参与以假设为导向和以探究为基础的实验项目，并与课程项目相联系的沟通技能，学生提高了他们的逻辑思维、清晰写作和连贯发言的能力。该项目调整和实施了一些研究，并以此为基础，这些研究表明需要并有效地将研究纳入本科课程(Brown，1988和Kenny，1998)。所描述的模块采用和实现了研究文章中概述的技术，这些研究文章引用了绿色荧光蛋白作为标记和研究工具的易用性(Misteli，1997和Conn，1999)。