SBIR Phase I: Direct Fluorescence Analysis by Low Temperature Time-Resolved Excitation Emission Matrices

SBIR 第一阶段：通过低温时间分辨激发发射矩阵进行直接荧光分析

• 批准号: 9960722
• 负责人: Greg Gillispie
• 金额: $ 10万
• 依托单位: DAKOTA TECHNOLOGIES INC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9960722&HistoricalAwards=false
• 关键词: SBIR; Phase; Direct; Fluorescence; Analysis

This Small Business Innovation Research Phase I project will lead to fast, sensitive, and compound-specific analysis of polycyclic aromatic hydrocarbons (PAHs) in air, water, soil, and sediment. Risk assessment demands chemically-specific analysis because the carcinogenic or mutagenic potential varies widely for PAHs with nearly identical chemical structures. The traditional chromatographic methods (GC or HPLC, often with mass detection) are slow and expensive. Fluorescence is amply sensitive, direct reading, and the data are inherently multi-dimensional. Prior research has shown that cooling Shpol'skii matrix samples to cryogenic temperature, exciting the fluorescence with narrow band tunable laser light, and time-resolved spectroscopy each enhance specificity. However, technical challenges have prevented the routine utilization of these strategies individually, let alone in combination. This project will demonstrate that benzo[a]pyrene can be quickly analyzed in real-world samples by time-resolved excitation emission matrices acquired for Shpol'skii matrices at 77 K. Technical innovations central to the project are an extremely compact tunable ultraviolet laser, simplification of fluorescence lifetime methodology, and delivering the excitation light directly to the sample by a fiber optic frozen into the matrix. The new procedures and instrumentation will see applications in environmental site characterization and remediation, health research, and fundamental laboratory studies.The initial commercial market is expected to be environmental testing and analytical laboratories. Once the feasibility of the TREEM direct fluorescence analysis is established, expansion to other markets, particularly in the pharmaceutical, agricultural and food industries is expected.

这个小企业创新研究第一阶段项目将导致对空气，水，土壤和沉积物中的多环芳烃（PAH）进行快速，灵敏和特定化合物的分析。风险评估需要化学分析，因为几乎相同化学结构的多环芳烃的致癌或致突变潜力差异很大。传统的色谱方法（GC或HPLC，通常带有质量检测）速度慢且昂贵。荧光是充分敏感的，直接阅读，并且数据固有地是多维的。先前的研究已经表明，将Shpol'skii基质样品冷却至低温，用窄带可调谐激光激发荧光，以及时间分辨光谱法各自增强特异性。 然而，技术上的挑战阻碍了这些战略的单独常规使用，更不用说组合使用了。该项目将证明，苯并[a]芘可以快速分析在真实世界的样品中获得的时间分辨的激发发射矩阵的Shpol'skii矩阵在77 K。该项目的核心技术创新是一个非常紧凑的可调谐紫外激光器，简化荧光寿命方法，并通过冻结到基质中的光纤将激发光直接传递到样品。新的程序和仪器将应用于环境场地表征和补救、健康研究和基础实验室研究。预计最初的商业市场将是环境测试和分析实验室。一旦确定了TREEM直接荧光分析的可行性，预计将扩展到其他市场，特别是制药，农业和食品行业。