Genetic Stocks of the Axolotl, Ambystoma Mexicanum

摘要该奖项为印第安纳大学蝾螈群落提供了新的资助，这是一个自给自足的墨西哥蝾螈（Ambystoma mexicanum）繁殖群体。该殖民地是一个遗传储备中心，致力于为美国和国外的实验室提供遗传特征良好的蝾螈胚胎，幼虫和成年。目前，IU殖民地每年向研究人员和教育工作者提供50,000只胚胎，幼虫，幼崽和成年动物。蝾螈是一种新生蝾螈，是一种非哺乳动物模型系统，具有许多在脊椎动物发育生物学、神经科学和免疫学实验研究中有利的特征。这些细胞包括显示灯刷染色体的大卵母细胞；适合于核移植的大的多精子卵子，可以很容易地获得单倍体或多倍体胚胎；大的、可接近的胚胎，能很好地耐受显微手术；大的单个神经细胞；以及肢体和器官再生的能力。

AbstractThis award provides renewed funding for the Indiana University Axolotl Colony, a self-sustaining, breeding colony of the Mexican axolotl (Ambystoma mexicanum). The Colony is a genetic stock center dedicated to the supply of genetically well-characterized axolotl embryos, larvae, and adults to laboratories throughout the United States and abroad. The IU Colony currently provides 50,000 embryonic, larval, juvenile and adult animals to researchers and educators each year.The axolotl, a neotenic salamander, is a non-mammalian model system with a number of features that are advantageous in experimental studies of vertebrate developmental biology, neuroscience, and immunology. These include large oocytes that display lampbrush chromosomes; large, polyspermic eggs that are suitable for nuclear transplantation and that can be easily manipulated to obtain haploids or polyploid embryos; large, accessible embryos that tolerate microsurgery well; large individual nerve cells; and the ability to regenerate limbs and organs.