Regulation of Cell Phospholipid Metabolism by Phospholipase A2

磷脂酶 A2 对细胞磷脂代谢的调节

• 批准号: 9982663
• 负责人: Suzanne Barbour
• 金额: $ 20.5万
• 依托单位: Virginia Commonwealth University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9982663&HistoricalAwards=false
• 关键词: Regulation; Cell; Phospholipid; Metabolism; Phospholipase

Phosphatidylcholine (PC) is the most abundant fat in the membranes that surround mammalian cells. This fat is absolutely essential for cell survival, as cells that cannot make PC are rapidly induced to undergo programmed cell death. Therefore, it is important for cells to maintain relatively constant levels of PC in their membranes. This is achieved through "homeostasis", a balance between the synthesis and degradation of PC. Many lines of evidence indicate that the synthesis of PC is controlled by a specific enzyme, CT. This research group has demonstrated that the degradation of PC may also be controlled by a specific enzyme, iPLA2. Therefore, experiments will be performed to determine if changes in the amount of PC in cell membranes induce compensatory changes in the amount of iPLA2 in mammalian cells. If this is the case, then the mechanism of the induction will be examined. Together, these studies should provide insight into a basic aspect of cell biology, the mechanisms that cells use to maintain their membranes.

磷脂酰胆碱（PC）是哺乳动物细胞膜中含量最丰富的脂肪。 这种脂肪对于细胞的生存是绝对必要的，因为不能制造PC的细胞会被迅速诱导进行程序性细胞死亡。 因此，重要的是细胞在其膜中保持相对恒定的PC水平。 这是通过“体内平衡”来实现的，即PC的合成和降解之间的平衡。 许多证据表明，PC的合成是由一种特定的酶CT控制的。 该研究小组已经证明，PC的降解也可能由一种特定的酶iPLA 2控制。 因此，将进行实验以确定细胞膜中PC量的变化是否诱导哺乳动物细胞中iPLA 2量的代偿性变化。 如果是这样的话，那么将检查诱导的机制。 总之，这些研究应该提供深入了解细胞生物学的一个基本方面，细胞用来维持其膜的机制。