International Fellow Awards: Microsystem 2 Dimensional Electrophoresis - Towards Rapid Separations for Proteomics and Diagnostics

0000462雷耶斯国际研究员奖计划使美国科学家和工程师能够在国外进行三到二十四个月的研究。 该计划的奖项提供了机会，联合研究，并使用独特的或互补的设施，专业知识和实验条件国外。雷耶斯在伦敦帝国理工学院与安德烈亚斯·曼兹博士合作了24个月，英国：该项目由国家科学基金会数学和物理科学理事会多学科活动办公室共同资助，研究目标是开发一种能够进行多重蛋白质分析-“蛋白质组阵列分析”-的分离系统，以分析基因组复杂性样本中的蛋白质水平。 它将涉及使用聚合物或石英基板上的微加工通道（伪凝胶），首先通过等电聚焦，然后通过十二烷基硫酸钠（SDS）凝胶电泳进行蛋白质的电泳分离。最终，该设备将用于分离复杂的多蛋白生物样品。Manz博士是用于化学应用的微芯片技术的先驱之一。 他参与了基于毛细管电泳、液相色谱和流动注射分析的高速分析仪的开发。*

0000462ReyesThe International Research Fellow Awards Program enables U.S. scientists and engineers to conduct three to twenty-four months of research abroad. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad.This award will provide Dr. Darwin R. Reyes with support for twenty-four months to work with Dr. Andreas Manz at Imperial College of Science in London, England.This project is co-funded by NSF's Directorate for Mathematical and Physical Sciences' Office of Multidisciplinary Activities.The goal of this research is to develop a separation system capable of multiplex protein analysis - `proteomic array analysis' - to analyze the levels of proteins in samples of genomic complexity. It will involve the use of microfabricated channels (pseudo-gels) on polymers or quartz substrates for electrophoretic separations of proteins, first by isoelectric focusing and then by sodium dodecyl sulfate (SDS) gel electrophoresis. Ultimately, the device will be employed to separate complex multiprotein biological samples.Dr. Manz is one of the pioneers in microchip technology used for chemical applications. He was involved in the development of high-speed analyzers based on capillary electrophoresis, liquid chromatography and flow injection analysis.***