International Fellow Awards: Microsystem 2 Dimensional Electrophoresis - Towards Rapid Separations for Proteomics and Diagnostics

[0000462] reyes国际研究员奖励计划使美国科学家和工程师能够在国外进行3至24个月的研究。该计划的奖励为联合研究提供了机会，并利用独特或互补的设施、专业知识和国外的实验条件。该奖项将为Darwin R. Reyes博士提供为期24个月的支持，让他与英国伦敦帝国理工学院的Andreas Manz博士一起工作。该项目由美国国家科学基金会数学和物理科学理事会多学科活动办公室共同资助。这项研究的目标是开发一种能够进行多重蛋白质分析的分离系统——“蛋白质组学阵列分析”——以分析基因组复杂性样品中的蛋白质水平。它将涉及在聚合物或石英底物上使用微制造通道（伪凝胶）进行蛋白质的电泳分离，首先通过等电聚焦，然后通过十二烷基硫酸钠（SDS）凝胶电泳。最终，该设备将用于分离复杂的多蛋白生物样本。Manz是将微芯片技术用于化学应用的先驱之一。他参与了基于毛细管电泳、液相色谱和流动注射分析的高速分析仪的开发

0000462ReyesThe International Research Fellow Awards Program enables U.S. scientists and engineers to conduct three to twenty-four months of research abroad. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad.This award will provide Dr. Darwin R. Reyes with support for twenty-four months to work with Dr. Andreas Manz at Imperial College of Science in London, England.This project is co-funded by NSF's Directorate for Mathematical and Physical Sciences' Office of Multidisciplinary Activities.The goal of this research is to develop a separation system capable of multiplex protein analysis - `proteomic array analysis' - to analyze the levels of proteins in samples of genomic complexity. It will involve the use of microfabricated channels (pseudo-gels) on polymers or quartz substrates for electrophoretic separations of proteins, first by isoelectric focusing and then by sodium dodecyl sulfate (SDS) gel electrophoresis. Ultimately, the device will be employed to separate complex multiprotein biological samples.Dr. Manz is one of the pioneers in microchip technology used for chemical applications. He was involved in the development of high-speed analyzers based on capillary electrophoresis, liquid chromatography and flow injection analysis.***