Modular proteins as organizers in the actin cytoskeleton: Integrators of functions

模块化蛋白作为肌动蛋白细胞骨架的组织者：功能整合者

• 批准号: 139304761
• 负责人: Professor Dr. Christoph S. Clemen
• 金额: --
• 依托单位: Institut für Luft- und Raumfahrtmedizin
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/139304761?language=en
• 关键词: Modular; proteins; organizers; actin; cytoskeleton

The use of modular proteins allows the involvement of these components in many different cellular reactions. The modules that have been defined in cytoskeletal proteins involve PH, SH3, PDZ and WD40 domains. Proteins containing such motifs form the basis of molecular complexes that organize cellular signalling and assemble components into functional units. Here, we concentrate on the coronin family of WD40 domain containing proteins. Mammalian and Dictyostelium discoideum short coronin proteins generally are important regulators of the actin cytoskeleton and influence cell migration, endocytosis and phagocytosis, and cytokinesis, while the single short yeast coronin CRNll additionally interacts with microtubules. Apart from that several coronins, particularly long coronins of the CRN7 subfamily, have been shown to participate in vesicular transport. Goal of the proposed studies is the analysis of the molecular mechanisms, interactions with cellular components, regulation of activity, and elucidation of the in vivo roles of coronin proteins using the mammalian short CRN2 and long CRN7 as well as Dictyostelium discoideum CRN7.

模组蛋白的使用允许这些组分参与许多不同的细胞反应。已在细胞骨架蛋白中定义的模块涉及PH、SH3、PDZ和WD40结构域。含有这种基序的蛋白质构成了分子复合体的基础，分子复合体组织细胞信号并将组件组装成功能单元。在这里，我们集中在含有WD40结构域的蛋白的冠状病毒家族。哺乳动物和盘基网眼菌的短冠蛋白通常是肌动蛋白细胞骨架的重要调节因子，影响细胞的迁移、内吞和吞噬以及胞质分裂，而单一的短曲霉菌短冠蛋白CRN11还与微管相互作用。除此之外，一些冠状蛋白，特别是CRN7亚家族的长冠状蛋白，已被证明参与囊泡运输。这项研究的目的是利用哺乳动物的短CRN2和长CRN7以及盘基网柄菌CRN7来分析冠状蛋白的分子机制、与细胞成分的相互作用、活性的调节以及阐明其在体内的作用。