Minority Postdoctoral Research Fellowships in Biological Sciences for FY 2000

2000财年生物科学少数族裔博士后研究奖学金

• 批准号: 0001969
• 负责人: Herschel Wade
• 金额: $ 10万
• 依托单位: Wade, Herschel V
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0001969&HistoricalAwards=false
• 关键词: Minority; Postdoctoral; Research; Fellowships; Biological

This action funds an NSF Minority Postdoctoral Research Fellowship in Biological Sciences for FY 2000.The research and training plan is in the area of biophysics and is entitled "Protein Engineering of a Model Protein." This study uses a synthetic diiron-oxo protein (DF) as a model for the class of metalloproteins that performs a variety of functions in living systems including dioxygen transport, conversion of ribonucleotides to deoxyribonucleotides, desaturation of fatty acids, and the solubilization and storage of iron. DF has been designed to serve as a minimal model of diiron-oxo proteins and has been shown to contain a metal center that is very similar to the diiron-oxo proteins. The model is interesting because the dinuclear metal centers in all diiron-oxo proteins contain many common structural features, but at the same time contain subtle differences that give rise to different activities. This makes the diiron-oxo system attractive for studying how protein environments can influence active site functions. This projects seeks to refine the first generation DF system into one that is amenable for engineering and designing efforts and for studying diiron-oxo protein function by (1) developing an efficient synthesis of DF which would offer a quick way to generate large numbers of DF variants and (2) by investigating the determinants for the function and structure of the center by systematically altering the atoms near the active site. Completing these goals will guide efforts towards designing pre-defined functions into the diiron-oxo centers.

这项行动资助了 2000 财年 NSF 生物科学少数博士后研究奖学金。该研究和培训计划属于生物物理学领域，题为“模型蛋白质的蛋白质工程”。 这项研究使用合成的二铁氧蛋白（DF）作为一类金属蛋白的模型，该金属蛋白在生命系统中执行多种功能，包括双氧运输、核糖核苷酸向脱氧核糖核苷酸的转化、脂肪酸的去饱和以及铁的溶解和储存。 DF 被设计作为二铁氧蛋白的最小模型，并且已被证明含有与二铁氧蛋白非常相似的金属中心。 该模型很有趣，因为所有二铁氧蛋白中的双核金属中心包含许多共同的结构特征，但同时包含引起不同活性的细微差异。 这使得二铁氧系统对于研究蛋白质环境如何影响活性位点功能具有吸引力。 该项目旨在通过以下方式将第一代 DF 系统细化为适合工程和设计工作以及研究二铁氧蛋白功能的系统：(1) 开发 DF 的有效合成方法，这将提供一种快速生成大量 DF 变体的方法；(2) 通过系统地改变活性位点附近的原子来研究中心功能和结构的决定因素。 完成这些目标将指导在二铁氧中心设计预定义功能的努力。