Postdoctoral Research Fellowship in Microbial Biology for FY 2000

这项行动为2000年国家科学基金会微生物生物学博士后研究金提供资金。研究和培训计划是在微生物遗传学领域，题为“微生物中的同源性机制”。 本研究探讨了超嗜热古菌Solfolobus solfataricus的一般重组机制。 真核重组蛋白的功能同源物正在异源产生，并用于在体外重建古细菌重组。 体内基因破坏和突变体拯救研究将补充体外数据，并证明由S.在酵母中产生的那些。同源重组是一个普遍的过程，其中遗传信息在DNA的不同链之间交换，引入新的连接和遗传序列。

This action funds an NSF Postdoctoral Research Fellowship in Microbial Biology for 2000.The research and training plan is in the area of Microbial Genetics and is entitled "Mechanism of Homologous Recombination in Archaea". This research examines general recombination mechanisms in the hyperthermophilic archaeal microbe Solfolobus solfataricus. Functional homologues to eukaryotic recombination proteins are being heterologously produced and are used to reconstitute archaeal recombination in-vitro. In-vivo gene disruption and mutant rescue studies will complement the in-vitro data and demonstrate functional homology of proteins produced by S. solfataricus to those produced in yeasts. Homologous recombination is a universal process in which genetic information is exchanged between separate strands of DNA, introducing new linkages and genetic sequences.