Postdoctoral Research fellowship in Microbial Biology for FY-2000

2000财年微生物学博士后研究奖学金

• 批准号: 0074374
• 负责人: Janet Lamb
• 金额: $ 10万
• 依托单位: Lamb, Janet R
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0074374&HistoricalAwards=false
• 关键词: Postdoctoral; Research; fellowship; Microbial; Biology

This action funds an NSF Postdoctoral Research Fellowship in Microbial Biology for 2000.The research and training plan is in the area of Regulation of Gene Expression and is entitled "Identification of Novel Pseudomonas aeruginosa Quorum Sensing Genes". Monitoring of surroundings by quorum sensing in P. aeruginosa, a gram-negative bacterium, plays an important role in its ability to grow in a wide variety of conditions. The two best studied quorum-sensing systems are the las and rhl systems. This research utilizes a fluorescence activated cell sorter (FACS)-based approach to identify novel genes positively and negatively regulated by the las and/or rhl system. Temporal induction or repression of the identified genes is examined during the growth cycle, and regulatory factors dictating the induction and/or repression of these genes are identified. In addition, the functions of these genes are examined via insertional activation and observation of the phenotypic changes.

这项行动资助了2000年美国国家科学基金会微生物生物学博士后研究奖学金。研究和培训计划是在基因表达调控领域，题为“新铜绿假单胞菌群体感应基因的鉴定”。 铜绿假单胞菌是一种革兰氏阴性菌，其群体感应对周围环境的监测对其在各种条件下的生长能力起着重要作用。 两个研究得最好的群体感应系统是las和rhl系统。 本研究利用荧光激活细胞分选仪（FACS）为基础的方法，以确定新的基因的积极和消极调节的las和/或rhl系统。 在生长周期中检查所识别基因的时间诱导或抑制，并识别决定这些基因的诱导和/或抑制的调节因子。 此外，通过插入激活和观察表型变化来检查这些基因的功能。