Postdoctoral Research Fellowships in Microbial Biology for FY 2000

2000财年微生物学博士后研究奖学金

• 批准号: 0074392
• 负责人: Alexandra Worden
• 金额: $ 10万
• 依托单位: Worden, Alexandra Z
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0074392&HistoricalAwards=false
• 关键词: Postdoctoral; Research; Fellowships; Microbial; Biology

This action funds an NSF Postdoctoral Research Fellowship in Microbial Biology for 2000.The research and training plan is in the area of Ecological and Evolutionary Physiology and is entitled "Diversity and Physiology of Extemely Small Algae." This research addresses the question of what role differences in niche adaptation play in the existence of different clades of marine cyanobacteria. Two free-living strains of the marine cyanobacterium Synechococcus, one representing a coastal clade and the other an open ocean clade, are being compared to assess physiological differences and adaptive capacities to nitrogen starvation. Subtraction cDNA libraries are being produced and the sequences analyzed to identify protein sequence and motifs of nitrate transporting proteins. Generated sequence is used to produce a microarray, and chip experiments are designed to examine and compare the magnitude and kinetics of the two strains to nitrogen deprivation. This data will help answer the question of whether different strains differ in expressional patterns in ways that indicate niche adaptation or resource partitioning, both of which are frequent evolutionary responses to competition.

该行动资助了2000年美国国家科学基金会微生物生物学博士后研究奖学金。这项研究和培训计划是在生态和进化生理学领域，题为“极小藻类的多样性和生理学”。本研究解决了在海洋蓝藻的不同分支的存在中，生态位适应的差异所起的作用。两种自由生活的海洋蓝藻聚球菌菌株，一种代表沿海分支，另一种代表开阔的海洋分支，正在进行比较，以评估生理差异和对氮饥饿的适应能力。目前正在建立减法cDNA文库，并对其序列进行分析，以确定硝酸盐转运蛋白的蛋白质序列和基序。生成的序列用于生产微阵列，并设计芯片实验来检查和比较两种菌株对氮剥夺的幅度和动力学。这一数据将有助于回答这样一个问题，即不同品系在表达模式上的差异是否表明了生态位适应或资源分配，这两种方式都是对竞争的常见进化反应。