Structural Determinants of Leghemoglobin Reactivity
豆血红蛋白反应性的结构决定因素
基本信息
- 批准号:0077890
- 负责人:
- 金额:$ 36万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2000
- 资助国家:美国
- 起止时间:2000-07-01 至 2003-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
HargroveMCB 0077890The long-range goal of this research is to develop a more comprehensive understanding of the structural and biophysical mechanisms, which regulate ligand binding to hemoglobins. The immediate goals are to use leghemoglobin as a complementary model system to test hypotheses for hemoglobin function, which are based heavily on the understanding of myoglobin. This work involves the use of site-directed mutagenesis, X-ray crystallography, and modern methods for the analysis of heme vibrational modes in combination with traditional kinetic and spectroscopic techniques to analyze the relationship of structure and function in soybean leghemoglobin. It has been demonstrated that this protein exploits different mechanisms for regulating ligand binding than those used by myoglobin. Therefore, a comprehensive study of leghemoglobin will undoubtedly provide new insight into hemoglobin structure and function. Heme proteins carry out a variety of important physiological functions. A basic understanding of monomeric heme proteins provides a starting point for investigating the structure and function of these different systems and allows for rational design to incorporate desired characteristics into better understood hemoglobins. It is not clear to what degree the current hypothesized mechanisms for hemoglobin regulation can be transferred to an understanding of other heme proteins. The research carried out through this project will test the current hypotheses for hemoglobin function using a comprehensive analysis of a monomeric hemoglobin, soybean leghemoglobin. A better understanding of ligand binding generated from a detailed study of this protein will complement our understanding of these processes gained from traditional model systems. The goals are to provide a more diverse and complete basis for exploring the same questions about more complex, multimeric hemoglobins, oxygen sensors, peroxidases, and related enzymes.
HargroveMCB 0077890这项研究的长期目标是更全面地了解调节配体与血红蛋白结合的结构和生物物理机制。 近期目标是使用豆血红蛋白作为补充模型系统来测试血红蛋白功能的假设,这些假设很大程度上基于对肌红蛋白的理解。 这项工作涉及使用定点突变、X 射线晶体学和现代血红素振动模式分析方法,结合传统动力学和光谱技术来分析大豆豆血红蛋白的结构和功能的关系。 已经证明,该蛋白质利用与肌红蛋白所使用的机制不同的机制来调节配体结合。 因此,对豆血红蛋白的全面研究无疑将为血红蛋白的结构和功能提供新的见解。 血红素蛋白具有多种重要的生理功能。 对单体血红素蛋白的基本了解为研究这些不同系统的结构和功能提供了起点,并允许合理设计将所需特征纳入更好理解的血红蛋白中。 目前尚不清楚当前假设的血红蛋白调节机制可以在多大程度上转化为对其他血红素蛋白的理解。 通过该项目进行的研究将通过对单体血红蛋白(大豆豆血红蛋白)的综合分析来测试当前关于血红蛋白功能的假设。 通过对该蛋白质的详细研究产生的配体结合的更好理解将补充我们对从传统模型系统获得的这些过程的理解。 目标是为探索有关更复杂的多聚血红蛋白、氧传感器、过氧化物酶和相关酶的相同问题提供更加多样化和完整的基础。
项目成果
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Mark Hargrove其他文献
P7 - STRUCTURE AND REACTIVITY OF HEXACOORDINATE HEMOGLOBINS
- DOI:
10.1016/j.freeradbiomed.2013.10.401 - 发表时间:
2013-11-01 - 期刊:
- 影响因子:
- 作者:
Mark Hargrove;Ryan Sturms - 通讯作者:
Ryan Sturms
Mark Hargrove的其他文献
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