Structures of Gas-Phase Protein Ions

这个名为“气相蛋白质离子结构”的研究项目是对印第安纳大学布卢明顿分校大卫·克莱默博士先前工作的重大扩展，并得到了分析和表面化学项目的支持。本研究的目的是利用改进的离子迁移率/飞行时间质谱仪研究气相蛋白质和肽的三维构象。所使用的仪器可以容纳大量的分析，这将使筛选大型化合物库成为可能。化学探针，如氢-氘（H/D）交换反应，将用于研究构象和反应性。该实验将氢能/氢能交换实验与碰撞诱导离解实验相结合，可能为解释和测量氢能/氢能交换数据提供一种重要的新技术。溶液相和气相蛋白质构象之间的相关性还没有很好地定义，可能与溶液相构象不相似。然而，如果发现相关性，该研究可能对理解生物反应性的化学机制以及蛋白质结构/反应性关系的研究产生重大影响。随着对蛋白质在生物系统中的作用的了解的增加，现在已经知道蛋白质的三维结构直接影响蛋白质的功能。因此，当今化学研究的一个重要领域是发展建立三维结构的技术。在克莱默教授的这项基础研究中，他将开发一种检测蛋白质结构的新技术。这项研究虽然风险很高，但通过提高对基于蛋白质结构/功能关系的疾病机制的理解，在未来有可能影响社会。这将导致药物开发和疾病治疗的加强。

This research program entitled "Structures of Gas-Phase Protein Ions" represents a significant expansion of the prior work of Dr. David Clemmer of Indiana University at Bloomington and is supported by the Analytical and Surface Chemistry program. The goal of this research is to investigate the 3-dimensional conformation of gas-phase proteins and peptides using a modified ion mobility/time of flight mass spectrometer. The instrumentation to be used can accommodate a high number of analyses, which will enable the screening of large libraries of compounds. Chemical probes, such as hydrogen-deuterium (H/D) exchange reactions, will be used to investigate both conformation and reactivity. The novel experiments planned, which couple H/D exchange studies with collision induced dissociation experiments, may result in an important new technique for interpreting and measuring H/D exchange data. The correlation between solution phase and gas phase protein conformations is not well defined, and may not be similar to solution phase conformations. However, if a correlation is found, this research could have a major impact on the understanding of the chemical mechanisms of biological reactivity and the study of protein structure/reactivity relationships. With the increased understanding of the role of proteins in biological systems, it is now known that the 3-dimensional structure of a protein directly effects the function to be performed by the protein. Thus, an important area of chemical research today is the development of techniques to establish the 3-dimensional structure. In this fundamental research by Professor Clemmer, he will develop a new technique to examine protein structure. This research, though high risk, has the potential to impact society in the future through improved understanding of disease mechanisms based on protein structure/function relationships. This will lead to enhancements in pharmaceutical development and treatment of disease.