Acquisition of Instrumentation for an Automated DNA Sequencing Facility

该奖项将支持在威斯康星州大学建立一个用于DNA序列分析的共同核心设施。 该设施的主要组成部分是一种最先进的仪器，用于确定任何生物体的DNA或RNA遗传物质中的核苷酸碱基序列。 参与的教师参与了与生物多样性和生物技术有关的广泛的研究项目。 主要项目包括：昆虫和哺乳动物痘病毒基因组的测序和突变分析;蓝藻和绿色藻类光合作用途径中涉及的基因分析;以及绘制寄生虫和真菌的进化历史。 这些项目将确定无数的新基因，并大大扩展对基因组组织的理解。 预计新的序列将增加我们对进化关系的理解，以及生物技术新产品的开发，如改进的植物工厂。 以下设备将成为新核心的一部分：LI-COR 4200 S-2 Gene ReadIR DNA测序系统，MJ Research PTC-0200 DNA引擎热循环仪和两台用于DNA分析的G4/450 Power Macintosh计算机。 共享设施旨在促进教育和研究的融合。测序操作的集中化将加强许多研究小组之间的现有互动，并将在吸引新的教师和学生攻读科学学位方面发挥重要作用。 它将促进与社区内其他机构的合作。 除了个人的研究计划，一些教师将开发实验室练习使用这种仪器，为本科生和硕士提供培训。通过将基因组学培训纳入生物技术实验室课程，系统生物学课程和联合免疫学/病毒学实验室课程等课程，为学生提供支持。

This award will support the creation of a common core facility for DNA sequence analysis at the University of Wisconsin. The main component of this facility is a state of the art instrument for determining the sequences of the nucleotide bases in the DNA or RNA genetic material for any organism. The participating faculty are involved in a broad range of research projects related to biodiversity and biotechnology. The main projects include: sequencing and mutational analysis of insect and mammalian poxvirus genomes; analysis of genes involved in the photosynthetic pathways of cyanobacteria and green algae; and charting the evolutionary history of parasites and fungi. These projects will identify countless new genes and greatly expand the understanding of genome organization. It is anticipated that the new sequences will inc rease our understanding of evolutionary relationships, and the development of new products in biotechnology such as improved plant factories. The following equipment will become part of the new core: a LI-COR 4200S-2 Gene ReadIR DNA sequencing system, a MJ Research PTC-0200 DNA engine thermocycler, and two G4/450 Power Macintosh computers for DNA analysis. The shared facility is aimed to foster the integration of education and research. The centralization of sequencing operations will enhance existing interactions among a number of research groups and will be vital in attracting new faculty and students to pursue degrees in the sciences. It will promote collaborations with other institutions within the community. Aside from individual research programs, several faculty members will develop laboratory exercises using this instrumentation to provide training for undergraduate and M.S. students by incorporating genomics training into such courses as the Biotechnology laboratory course, a Systematic Biology course, and the Combined Immunology/Virology laboratory course.