Acquisition of Instrumentation for an Automated DNA Sequencing Facility

该奖项将支持在威斯康星大学建立一个用于DNA序列分析的共同核心设施。该设备的主要组成部分是用于确定任何生物体DNA或RNA遗传物质中核苷酸碱基序列的最先进仪器。参与的教师参与了与生物多样性和生物技术有关的广泛的研究项目。主要项目包括：昆虫和哺乳动物痘病毒基因组测序和突变分析；蓝藻和绿藻光合途径相关基因分析绘制寄生虫和真菌的进化史。这些项目将识别出无数的新基因，并极大地扩展对基因组组织的理解。预计新的序列将增加我们对进化关系的理解，并在生物技术领域开发新产品，如改良植物工厂。以下设备将成为新核心的一部分：LI-COR 4200S-2 Gene ReadIR DNA测序系统，MJ Research PTC-0200 DNA引擎热循环器和两台用于DNA分析的G4/450 Power Macintosh计算机。共享设施旨在促进教育和研究的整合。测序操作的集中将加强若干研究小组之间现有的相互作用，并将对吸引新的教师和学生攻读科学学位至关重要。它将促进与社区内其他机构的合作。除了个人研究项目外，一些教师将使用该仪器开发实验室练习，通过将基因组学培训纳入诸如生物技术实验室课程、系统生物学课程和免疫学/病毒学联合实验室课程等课程，为本科生和硕士学生提供培训。

This award will support the creation of a common core facility for DNA sequence analysis at the University of Wisconsin. The main component of this facility is a state of the art instrument for determining the sequences of the nucleotide bases in the DNA or RNA genetic material for any organism. The participating faculty are involved in a broad range of research projects related to biodiversity and biotechnology. The main projects include: sequencing and mutational analysis of insect and mammalian poxvirus genomes; analysis of genes involved in the photosynthetic pathways of cyanobacteria and green algae; and charting the evolutionary history of parasites and fungi. These projects will identify countless new genes and greatly expand the understanding of genome organization. It is anticipated that the new sequences will inc rease our understanding of evolutionary relationships, and the development of new products in biotechnology such as improved plant factories. The following equipment will become part of the new core: a LI-COR 4200S-2 Gene ReadIR DNA sequencing system, a MJ Research PTC-0200 DNA engine thermocycler, and two G4/450 Power Macintosh computers for DNA analysis. The shared facility is aimed to foster the integration of education and research. The centralization of sequencing operations will enhance existing interactions among a number of research groups and will be vital in attracting new faculty and students to pursue degrees in the sciences. It will promote collaborations with other institutions within the community. Aside from individual research programs, several faculty members will develop laboratory exercises using this instrumentation to provide training for undergraduate and M.S. students by incorporating genomics training into such courses as the Biotechnology laboratory course, a Systematic Biology course, and the Combined Immunology/Virology laboratory course.