Identification of Pea Leaf Nuclear PGK, GAPD & Aldolase Isoforms

豌豆叶核PGK、GAPD的鉴定

• 批准号: 0079913
• 负责人: Louise Anderson
• 金额: $ 6.33万
• 依托单位: University of Illinois at Chicago
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0079913&HistoricalAwards=false
• 关键词: Identification; Pea; Leaf; Nuclear; PGK

Louise AndersonP-glycerate kinase, glyceraldehyde-3-P dehydrogenase, and aldolase, or their antigenic analogs, are present in the nucleus of pea leaf mesophyll cells. The kinase appears to be a subunit of the primer recognition protein involved in lagging strand synthesis. The dehydrogenase may be a part of this same complex and/or may have additional functions related to nucleotide binding. Aldolase might have a function related to DNA-binding. It is not known whether the nuclear proteins are the chloroplast Calvin cycle (reductive pentose phosphate pathway) isozymes or the cytosolic isozymes. The isozymes present in the nucleus will be identified using mono-specific, isozyme-specific antibodies. These experiments will be important to understanding the regulation of nucleic acid metabolism and gene expression in the plant nucleus and in the chloroplast. The antibodies will also be used in co-localization studies.The investigator has found three enzymes in the nucleus that appear to be the same as three enzymes found in the chloroplast or in the cytosol. The surprising aspect of this discovery is that the functions of the nuclear enzymes must differ markedly from those of their chloroplast and cytosolic counterparts, which are known to function in carbon metabolism. The investigator will rigorously compare and identify these enzymes from the different cellular areas.This work will lay the foundation for future studies of the possible roles of these nuclear enzymes in nuclear metabolism and gene regulation.

Louise AndersonP-甘油酸激酶、甘油醛-3-P脱氢酶和醛缩酶或其抗原类似物存在于豌豆叶叶肉细胞的细胞核中。 激酶似乎是参与滞后链合成的引物识别蛋白的亚基。 脱氢酶可以是该相同复合物的一部分和/或可以具有与核苷酸结合相关的额外功能。 醛缩酶可能具有与DNA结合相关的功能。 核蛋白是叶绿体卡尔文循环（还原戊糖磷酸途径）同工酶还是胞质同工酶还不清楚。 将使用单特异性、同工酶特异性抗体鉴定细胞核中存在的同工酶。 这些实验对于理解植物细胞核和叶绿体中核酸代谢和基因表达的调控具有重要意义。 研究人员在细胞核中发现了三种酶，它们似乎与叶绿体或细胞质中发现的三种酶相同。 这一发现令人惊讶的方面是，核酶的功能必须明显不同于叶绿体和胞质的对应物，这是已知的碳代谢功能。 研究人员将严格比较和鉴定来自不同细胞区域的这些酶，这项工作将为将来研究这些核酶在核代谢和基因调控中的可能作用奠定基础。