Identification of Pea Leaf Nuclear PGK, GAPD & Aldolase Isoforms

路易斯·安德森-甘油激酶、甘油醛-3- p脱氢酶和醛缩酶或它们的抗原类似物存在于豌豆叶叶肉细胞的细胞核中。该激酶似乎是参与滞后链合成的引物识别蛋白的一个亚基。脱氢酶可能是该复合体的一部分和/或可能具有与核苷酸结合相关的附加功能。醛缩酶可能具有与dna结合有关的功能。目前尚不清楚核蛋白是叶绿体卡尔文循环（还原戊糖磷酸途径）同工酶还是胞质同工酶。存在于细胞核中的同工酶可以用单特异性抗体和同工酶特异性抗体来鉴定。这些实验对了解植物细胞核和叶绿体中核酸代谢和基因表达的调控具有重要意义。这些抗体也将用于共定位研究。研究者在细胞核中发现了三种酶，它们似乎与叶绿体或细胞质中发现的三种酶相同。这一发现令人惊讶的方面是，核酶的功能与叶绿体和细胞质酶的功能明显不同，而叶绿体和细胞质酶在碳代谢中起作用。研究者将严格比较和鉴定这些酶来自不同的细胞区域。这项工作将为进一步研究这些核酶在核代谢和基因调控中的可能作用奠定基础。

Louise AndersonP-glycerate kinase, glyceraldehyde-3-P dehydrogenase, and aldolase, or their antigenic analogs, are present in the nucleus of pea leaf mesophyll cells. The kinase appears to be a subunit of the primer recognition protein involved in lagging strand synthesis. The dehydrogenase may be a part of this same complex and/or may have additional functions related to nucleotide binding. Aldolase might have a function related to DNA-binding. It is not known whether the nuclear proteins are the chloroplast Calvin cycle (reductive pentose phosphate pathway) isozymes or the cytosolic isozymes. The isozymes present in the nucleus will be identified using mono-specific, isozyme-specific antibodies. These experiments will be important to understanding the regulation of nucleic acid metabolism and gene expression in the plant nucleus and in the chloroplast. The antibodies will also be used in co-localization studies.The investigator has found three enzymes in the nucleus that appear to be the same as three enzymes found in the chloroplast or in the cytosol. The surprising aspect of this discovery is that the functions of the nuclear enzymes must differ markedly from those of their chloroplast and cytosolic counterparts, which are known to function in carbon metabolism. The investigator will rigorously compare and identify these enzymes from the different cellular areas.This work will lay the foundation for future studies of the possible roles of these nuclear enzymes in nuclear metabolism and gene regulation.