Biophotonics: Endoscopic Confocal Microscopy by Spectral Encoding

生物光子学：光谱编码内窥镜共焦显微镜

• 批准号: 0086709
• 负责人: Guillermo Tearney
• 金额: $ 27万
• 依托单位: Massachusetts General Hospital
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0086709&HistoricalAwards=false
• 关键词: Biophotonics; Endoscopic; Confocal; Microscopy; Spectral

0086709TearneyThe objective of this program is to develop and construct a prototype clinical confocal microscopy system capable of non-invasively obtaining images of cellular microstructure in internal human organ systems. The scanning techniques currently used in laser confocal microscopy are not readily adaptable to the dimensions and constraints of medical endoscopes. Spectrally encoded confocal microscopy (SECM), a technique developed in this laboratory, uses a broad bandwidth light source and a diffraction grating to simultaneously illuminate multiple points along a transverse line within a sample and to detect reflected light from these points. Since this method does not require fast spatial scanning mechanisms within the probe, it is capable of being miniaturized and incorporated into a catheter or endoscope. Special emphasis will be placed on developing an endoscopic confocal microscopy imaging system that is clinically viable, and can potentially be applied to applications ranging from screening for dysplasia to intra-operative tumor margin detection. In addition, this imaging modality could be used for basic science applications to investigate the pathophysiology of disease processes at a cellular level in situ.

该项目的目标是开发和构建一个临床共聚焦显微镜系统原型，该系统能够无创地获取人体内部器官系统的细胞微观结构图像。目前在激光共聚焦显微镜中使用的扫描技术不容易适应医学内窥镜的尺寸和限制。光谱编码共聚焦显微镜（SECM）是该实验室开发的一项技术，它使用宽带宽光源和衍射光栅同时照亮样品内沿横线的多个点，并检测这些点的反射光。由于该方法不需要探针内的快速空间扫描机制，因此它能够小型化并集成到导管或内窥镜中。特别的重点将放在发展一种内镜共聚焦显微镜成像系统，是临床可行的，可以潜在地应用于从筛查异常增生到术中肿瘤边缘检测的应用范围。此外，这种成像方式可用于基础科学应用，在细胞水平上原位研究疾病过程的病理生理学。