Biophotonics: Endoscopic Confocal Microscopy by Spectral Encoding

0086709Tearney本项目的目的是开发和构建一个原型临床共聚焦显微镜系统，能够非侵入性地获得人体内部器官系统中细胞微观结构的图像。目前在激光共聚焦显微镜中使用的扫描技术不容易适应医疗内窥镜的尺寸和限制。 光谱编码共焦显微镜（SECM），在这个实验室开发的技术，使用宽带光源和衍射光栅，同时照亮多个点沿着一个横向线内的样品，并检测从这些点的反射光。 由于该方法不需要探头内的快速空间扫描机构，因此能够小型化并结合到导管或内窥镜中。 特别强调将放在开发一个内窥镜共聚焦显微镜成像系统，是临床上可行的，并有可能被应用于从筛查异型增生的手术中肿瘤边缘检测的应用。 此外，这种成像模式可用于基础科学应用，以原位细胞水平研究疾病过程的病理生理学。

0086709TearneyThe objective of this program is to develop and construct a prototype clinical confocal microscopy system capable of non-invasively obtaining images of cellular microstructure in internal human organ systems. The scanning techniques currently used in laser confocal microscopy are not readily adaptable to the dimensions and constraints of medical endoscopes. Spectrally encoded confocal microscopy (SECM), a technique developed in this laboratory, uses a broad bandwidth light source and a diffraction grating to simultaneously illuminate multiple points along a transverse line within a sample and to detect reflected light from these points. Since this method does not require fast spatial scanning mechanisms within the probe, it is capable of being miniaturized and incorporated into a catheter or endoscope. Special emphasis will be placed on developing an endoscopic confocal microscopy imaging system that is clinically viable, and can potentially be applied to applications ranging from screening for dysplasia to intra-operative tumor margin detection. In addition, this imaging modality could be used for basic science applications to investigate the pathophysiology of disease processes at a cellular level in situ.