LTER Cross-Site: Bacterial Community Composition and Dissolved Organic Matter in Streams: A Cross Site Comparison

主要研究者：Leff，LauraDEB-0087051“溪流中的细菌群落组成和溶解有机物：跨站点比较“关于细菌群落分类组成和种群生态学的信息通常缺乏。 本研究将鉴定和比较不同数量和来源的溶解性有机物（DOM）的溪流中的细菌群落组成。 PI假设DOM来源不同的溪流将具有不同的细菌群落组成，并且来自不同溪流的细菌对不同类型DOM的实验暴露可以深刻地影响溪流的细菌群落，并且细菌对不同DOM混合物的反应不同。 该项目有两个组成部分：实地调查和实验室实验。 实地工作将被用来证明细菌多样性的广度和溪流之间的差异。实验将被用来证明DOM的差异所造成的流中观察到的模式。 实地调查将在11个溪流中进行：6个在LTER站点（Konza，Kellog，Coweeta和North Temperate Lakes），其他则根据DOM特征进行选择。 这项研究将允许细菌多样性信息添加到LTER信息库，并回答有关控制微生物多样性的因素的基本问题。 在许多情况下，迫使生态学家“黑箱”细菌群落的方法学限制将被本领域和相关工作所克服。 这为其他基本问题奠定了基础，这些问题涉及多样性在生态系统功能中的作用以及人类活动对多样性的危害程度。

PI: Leff, LauraDEB-0087051"Bacterial community composition and dissolved organic matter in streams: a cross site comparison"Information about bacterial community taxonomic composition and population ecology is generally lacking. This study will identify and compare bacterial community composition among streams with different amounts and sources of dissolved organic matter (DOM). The PI hypothesizes that streams that differ in the source of DOM will have different bacterial community composition and that experimental exposure of bacteria from different streams to different types of DOM can profoundly affect the bacterial community of streams and that bacteria differ in their responses to different DOM mixtures. The project has two components: a field survey and lab experiments. Field work will be used to demonstrate the breadth of bacterial diversity and the differences among streams. Experiments will be used to demonstrate of DOM differences caused the patterns observed in the streams. The field survey will be done in 11 streams: six at LTER sites (Konza, Kellog, Coweeta, and North Temperate Lakes) and the others selected based on their DOM features. This study will allow the addition of bacterial diversity information to the LTER information base and answer basic questions about factors that control microbial diversity. Methodological limitations that have forced ecologists, in many situations, to "black box" bacterial communities will be overcome by this are and related work. This lays the groundwork for other fundamental questions about the role diversity plays in ecosystem function and the degree to which it is imperiled by human activities.