LTER Cross-Site: Bacterial Community Composition and Dissolved Organic Matter in Streams: A Cross Site Comparison

PI: Leff, lauradebb -0087051“河流中细菌群落组成和溶解有机质：一个跨站点的比较”细菌群落的分类组成和种群生态学的信息普遍缺乏。本研究将确定和比较不同数量和来源的溶解有机物（DOM）溪流的细菌群落组成。PI假设不同DOM来源的河流会有不同的细菌群落组成，不同河流的细菌实验暴露于不同类型的DOM会深刻影响河流的细菌群落，细菌对不同DOM混合物的反应也不同。这个项目有两个组成部分：实地调查和实验室实验。实地工作将用于证明细菌多样性的广度和溪流之间的差异。实验将用于演示在流中观察到的模式所导致的DOM差异。实地调查将在11条河流中进行：6条在LTER站点（Konza, Kellog， Coweeta和北温带湖泊），其他的根据其DOM特征选择。本研究将允许在LTER信息库中添加细菌多样性信息，并回答有关控制微生物多样性因素的基本问题。在许多情况下，方法上的限制迫使生态学家“黑盒”细菌群落，这将被这项工作和相关工作所克服。这为其他关于多样性在生态系统功能中所起的作用及其受到人类活动危害的程度的基本问题奠定了基础。

PI: Leff, LauraDEB-0087051"Bacterial community composition and dissolved organic matter in streams: a cross site comparison"Information about bacterial community taxonomic composition and population ecology is generally lacking. This study will identify and compare bacterial community composition among streams with different amounts and sources of dissolved organic matter (DOM). The PI hypothesizes that streams that differ in the source of DOM will have different bacterial community composition and that experimental exposure of bacteria from different streams to different types of DOM can profoundly affect the bacterial community of streams and that bacteria differ in their responses to different DOM mixtures. The project has two components: a field survey and lab experiments. Field work will be used to demonstrate the breadth of bacterial diversity and the differences among streams. Experiments will be used to demonstrate of DOM differences caused the patterns observed in the streams. The field survey will be done in 11 streams: six at LTER sites (Konza, Kellog, Coweeta, and North Temperate Lakes) and the others selected based on their DOM features. This study will allow the addition of bacterial diversity information to the LTER information base and answer basic questions about factors that control microbial diversity. Methodological limitations that have forced ecologists, in many situations, to "black box" bacterial communities will be overcome by this are and related work. This lays the groundwork for other fundamental questions about the role diversity plays in ecosystem function and the degree to which it is imperiled by human activities.