LTER Cross-Site: Bacterial Community Composition and Dissolved Organic Matter in Streams: A Cross Site Comparison

PI：Lef，LauraDEB-0087051《溪流中细菌群落组成和溶解有机物：跨地点比较》关于细菌群落分类组成和种群生态的信息普遍缺乏。这项研究将识别和比较不同溶解有机质(DOM)数量和来源的溪流中的细菌群落组成。PI假设，不同DOM来源的溪流将具有不同的细菌群落组成，不同溪流的细菌暴露于不同类型的DOM可以深刻影响溪流的细菌群落，并且细菌对不同DOM混合物的反应不同。该项目包括两个部分：实地调查和实验室实验。现场工作将被用来展示细菌多样性的广度和溪流之间的差异。实验将被用来演示在河流中观察到的模式导致的DOM差异。实地调查将在11个河流中进行：6个在LTER地点(Konza、Kellog、Coweeta和北温带湖泊)，其他根据其DOM特征选择。这项研究将允许将细菌多样性信息添加到LTER信息库，并回答有关控制微生物多样性的因素的基本问题。在许多情况下，迫使生态学家“黑箱”细菌群落的方法限制将被这项研究和相关工作所克服。这为生物多样性在生态系统功能中的作用以及人类活动对其的危害程度等其他基本问题奠定了基础。

PI: Leff, LauraDEB-0087051"Bacterial community composition and dissolved organic matter in streams: a cross site comparison"Information about bacterial community taxonomic composition and population ecology is generally lacking. This study will identify and compare bacterial community composition among streams with different amounts and sources of dissolved organic matter (DOM). The PI hypothesizes that streams that differ in the source of DOM will have different bacterial community composition and that experimental exposure of bacteria from different streams to different types of DOM can profoundly affect the bacterial community of streams and that bacteria differ in their responses to different DOM mixtures. The project has two components: a field survey and lab experiments. Field work will be used to demonstrate the breadth of bacterial diversity and the differences among streams. Experiments will be used to demonstrate of DOM differences caused the patterns observed in the streams. The field survey will be done in 11 streams: six at LTER sites (Konza, Kellog, Coweeta, and North Temperate Lakes) and the others selected based on their DOM features. This study will allow the addition of bacterial diversity information to the LTER information base and answer basic questions about factors that control microbial diversity. Methodological limitations that have forced ecologists, in many situations, to "black box" bacterial communities will be overcome by this are and related work. This lays the groundwork for other fundamental questions about the role diversity plays in ecosystem function and the degree to which it is imperiled by human activities.