Regulation of Inflorescence and Pedicel Development in Tomato.

有花植物的茎分生组织是一个多细胞器官，为茎的无限生长提供能力。 研究小组将通过继续我们对番茄无关节-1突变的研究，调查茎分生组织及其衍生器官中的细胞在发育过程中是如何协调的。 在无节-1突变体中，花序在仅形成一朵或两朵花后恢复营养生长，并以旺盛的叶状枝终止。jointless-1突变体也形成完全缺乏分离区的花梗。 因此，番茄的JOINTLESS-1基因参与两个重要的发育过程，花序分生组织状态的维持和花梗分裂区的形成。 通过分析平周嵌合体，发现分生组织的内部（L3）细胞控制花序分生组织的确定性和花梗分裂区的形成。 克隆了JOINTLESS-1基因，发现它是一个MADS盒基因。对花序分生组织维持和花梗分裂带发育过程中细胞协调性的研究将继续进行。 1.原位杂交将进行使用JOINTLESS-1探针野生型和无关节-1突变体和6个平周嵌合体，以了解更多关于细胞协调的性质，发生在花序发育和分裂区形成。 2. 我们将在各种突变体中研究JOINTLESS-1的表达模式，我们发现这些突变体显示出与来自双突变体分析的jointless-1的遗传相互作用。 这些研究将提供一个更清楚的了解这些基因的相互作用，导致不同类型的分生组织活动。3.将在野生型植物和macrocalyx（另一种影响分裂区发育和花序分生组织维持的突变）之间以及macrocalyx和jointless-1之间构建平周嵌合体。 将测试大萼参与接收由L3细胞产生的信号的假设，L3细胞是无关节-1基因的野生型。 4. 将鉴定发育中的花梗的谱系分析，以确定在野生型和无节-1突变体植物中产生该器官的各个区域（近端花梗、分裂区和远端花梗）的细胞分裂模式。 通过这些实验，应该更多地了解细胞和遗传的相互作用，调节分裂区的形成和花序发育，以阐明这两个发展有趣的和农业上重要的过程之间的关系。

The shoot meristem of flowering plants is a multicellular organ that provides the capacity for indeterminate growth of the shoot. The research team will investigate how the cells in the shoot meristem and its derivative organs are coordinated during development by continuing our study of the jointless-1 mutation of tomato. In jointless-1 mutants, the inflorescences revert to vegetative growth after forming only one or two flowers and terminate with a vigorous, leafy shoot. jointless-1 mutants also form flower pedicels that completely lack an abscission zone. Thus, the JOINTLESS-1 gene of tomato is involved in two important developmental processes, the maintenance of the inflorescence meristem state and the formation of the pedicel abscission zone. By analyzing periclinal chimeras it was found that internal (L3) cells of the meristem control both inflorescence meristem determinacy and pedicel abscission zone formation. Cloning of JOINTLESS-1 revealed that it is a MADS box gene. The investigations of coordination of cells during the processes of maintaining the inflorescence meristem and development of pedicel abscission zones will be continued. 1. In situ hybridization will be performed using JOINTLESS-1 probes on wild-type and jointless-1 mutants and on the six periclinal chimeras to learn more about the nature of the cell coordination that occurs during inflorescence development and abscission zone formation. 2. Patterns of JOINTLESS-1 expression will be examined in various mutants that we have found to show genetic interactions with jointless-1 from double mutant analysis. Such studies will provide a clearer understanding of the interactions of these genes that result in different types of meristem activity. 3. Sets of periclinal chimeras will be constructed between wild-type plants and macrocalyx, another mutation that affects both abscission zone development and inflorescence meristem maintenance, and between macrocalyx and jointless-1. The hypothesis that macrocalyx is involved in the reception of the signal produced by L3 cells that are wild-type for the JOINTLESS -1 gene will be tested. 4. A lineage analysis will be identified for the developing pedicel to determine the patterns of cell divisions that give rise to the various regions of this organ (proximal pedicel, abscission zone, and distal pedicel) in wild-type and jointless-1 mutant plants. With these experiments more should be learned about the cellular and genetic interactions regulating abscission zone formation and inflorescence development to elucidate the relationship between these two developmentally interesting and agriculturally important processes.