Function of Juvenile Hormone Esterase Binding Protein in Insects

昆虫保幼激素酯酶结合蛋白的功能

• 批准号: 0090874
• 负责人: Bryony Bonning
• 金额: $ 31.79万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0090874&HistoricalAwards=false
• 关键词: Function; Juvenile; Hormone; Esterase; Binding

Juvenile hormone esterase (JHE) helps to regulate the titer of juvenile hormone and, hence, larval development in insects. JHE is actively removed from hemolymph by athrocytes that maintain appropriate hemolymph composition, and is degraded in lysosomes. A binding protein, P29, binds JHE in athrocytes and appears to be important for appropriate processing of JHE in these cells. Dr. Bonning aims to identify the mechanism(s) by which P29 affects the trafficking and degradation of JHE. To do this she will use both the tobacco hornworm Manduca sexta and the fruit fly Drosophila melanogaster in experiments designed to determine the intracellular location of P29, and the location of interaction of P29 with JHE. The specific functions of P29 with respect to JHE will then be elucidated by using mutant lines of D. melanogaster and RNA interference to prevent the interaction of the two proteins. The effects of disruption of P29 binding to JHE on the clearance and turnover of JHE will be observed. New knowledge about the nature and function of P29 will enhance our knowledge of protein trafficking and could facilitate development of novel approaches to insect pest control.

保幼激素酯酶(JHE)有助于调节昆虫保幼激素的滴度，从而调节幼虫的发育。JHE被维持适当血淋巴成分的促红细胞主动从血淋巴中清除，并在溶酶体中降解。一种名为P29的结合蛋白与甲状腺细胞中的JHE结合，似乎对这些细胞中JHE的适当处理至关重要。邦宁博士的目标是确定P29影响JHE贩运和退化的机制(S)。为了做到这一点，她将使用烟草角虫Manduca sexta和果蝇黑腹果蝇进行实验，以确定P29的细胞内位置，以及P29与JHE相互作用的位置。P29与JHE相关的特定功能将通过使用黑腹果蝇突变株和RNA干扰来阐明，以防止这两种蛋白质的相互作用。将观察破坏P29与JHE结合对JHE清除和周转的影响。有关P29的性质和功能的新知识将加强我们对蛋白质运输的了解，并可能促进开发新的害虫控制方法。