Function of Juvenile Hormone Esterase Binding Protein in Insects

幼体激素酯酶（JHE）有助于调节幼体激素的滴度，从而调节昆虫的幼虫发育。JHE被保持适当血淋巴成分的粥样细胞主动地从血淋巴中清除，并在溶酶体中降解。一种结合蛋白，P29，在动脉粥样硬化细胞中结合JHE，似乎对这些细胞中JHE的适当加工很重要。Bonning博士旨在确定P29影响JHE贩运和降解的机制。为此，她将使用烟草角虫Manduca sexta和果蝇Drosophila melanogaster进行实验，以确定P29在细胞内的位置，以及P29与JHE相互作用的位置。P29对JHE的具体功能将通过使用黑腹菌的突变系和RNA干扰来阐明。我们将观察P29与JHE结合的破坏对JHE的清除和周转的影响。关于P29的性质和功能的新知识将增强我们对蛋白质转运的认识，并有助于开发新的害虫防治方法。

Juvenile hormone esterase (JHE) helps to regulate the titer of juvenile hormone and, hence, larval development in insects. JHE is actively removed from hemolymph by athrocytes that maintain appropriate hemolymph composition, and is degraded in lysosomes. A binding protein, P29, binds JHE in athrocytes and appears to be important for appropriate processing of JHE in these cells. Dr. Bonning aims to identify the mechanism(s) by which P29 affects the trafficking and degradation of JHE. To do this she will use both the tobacco hornworm Manduca sexta and the fruit fly Drosophila melanogaster in experiments designed to determine the intracellular location of P29, and the location of interaction of P29 with JHE. The specific functions of P29 with respect to JHE will then be elucidated by using mutant lines of D. melanogaster and RNA interference to prevent the interaction of the two proteins. The effects of disruption of P29 binding to JHE on the clearance and turnover of JHE will be observed. New knowledge about the nature and function of P29 will enhance our knowledge of protein trafficking and could facilitate development of novel approaches to insect pest control.