Analysis of the Drosophila Gene Rhomboid

果蝇菱形基因分析

• 批准号: 0094634
• 负责人: Ethan Bier
• 金额: $ 42万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2004-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0094634&HistoricalAwards=false
• 关键词: Analysis; Drosophila; Gene; Rhomboid

0094634BierDr. Bier intends to study the role of a multi-pass integral membrane protein known as Rhomboid (Rho) in promoting signaling via the Epidermal Growth Factor Receptor (EGF-R) in the fruit fly Drosophila melanogaster. The EGF-R is a member of the widely used receptor tyrosine kinase (RTK) family, which activates the RAS/MAP-Kinase pathway. Rhomboid functions in conjunction with a single pass transmembrane protein (Star) to activate EGF-R signaling by a mechanism involving the production of a diffusible extracellular signal. The specific goals of this project are to: 1) analyze the function of domains of Rho that are predicted to lie inside or outside of the cell, 2) examine the basis for the intimate interaction between Rho and Star in promoting EGF-R signaling, and 3) determine whether Rho and Star participate directly or indirectly in producing a diffusible EGF-like signal. Dr. Bier anticipates that this study will lead to the formulation of specific mechanistic models for how Rho and Star activate EGF-ligands. More generally, this project should broaden our understanding of fundamental mechanisms for regulating the production and range of action of RTK ligands during development. Since RTK signaling pathways play a central role in development and regulation of cellular proliferation in vertebrates and invertebrates alike, these insights should have general implications for understanding cell-cell communication, which is also highly

0094634BierDr. Bier打算研究一种称为Rhomboid（Rho）的多通道整合膜蛋白在果蝇中通过表皮生长因子受体（EGF-R）促进信号传导的作用。 EGF-R是广泛使用的受体酪氨酸激酶（RTK）家族的成员，可激活RAS/MAP-激酶途径。 菱形蛋白与单次跨膜蛋白（星星）一起起作用，通过涉及产生可扩散的细胞外信号的机制激活EGF-R信号传导。 本项目的具体目标是：1）分析预测位于细胞内或细胞外的Rho结构域的功能，2）检查Rho和星星在促进EGF-R信号传导中的密切相互作用的基础，和3）确定Rho和星星是否直接或间接参与产生可扩散的EGF样信号。 Bier博士预计，这项研究将导致Rho和星星如何激活EGF配体的具体机制模型的制定。 更一般地说，这个项目应该扩大我们的理解的基本机制，调节生产和RTK配体的行动范围在发展过程中。 由于RTK信号通路在脊椎动物和无脊椎动物的细胞增殖的发育和调节中起着核心作用，这些见解应该对理解细胞间通讯具有普遍意义，细胞间通讯也是非常重要的。