SBIR Phase I: A Near-Instantaneous, Whole Blood Immunoassay

SBIR 第一阶段：近乎瞬时的全血免疫测定

• 批准号: 0109821
• 负责人: Daniel Watkins
• 金额: $ 10万
• 依托单位: NANOSPECTRA BIOSCIENCES, INC.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2001-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0109821&HistoricalAwards=false
• 关键词: SBIR; Phase; Near; Instantaneous; Whole

This Small Business Technology Transfer Phase 1 Project (SBIR) will contribute towards the development of a near-instantaneous, all-optical biosensing technology to replace ELISA-type (enzyme-linked immunoadsorbant assay) assays. Utilizing the unique optical properties of Metal Nanoshells, a new type of nanoparticle containing a dielectric core coated with a thin metal layer, immunoassays can be performed in the near-infrared region of high physiological transmissivity (wavelengths between 800 and 1300 nm) using Surface Enhanced Raman Scattering techniques. The equipment required to perform this immunoassay will be both highly portable and inexpensive. Initial research with a model antigen has shown that the nanoshell-based assay can produce results on whole-blood samples in 20 seconds. This is quantitatively equivalent to ELISA results requiring 24 to 48 hours. The proposed research will investigate the effects of bioconjugation of clinically important antibodies onto the nanoshell surface and examination of multiple Raman dyes for multi-antigen/analyte assays .The primary commercial application of the technology being developed in this project is in the $20 billion immunoassay market. The proposed research could lead to rapid immunoassay devices for ambulances, military and civilian health agencies, point-of-care analysis and high volume pharmaceutical testing. The core technology may have additional commercial applications in the area of biochips, in genomics and proteomics research, and in animal biology

该小型企业技术转让第1阶段项目（SBIR）将有助于开发一种近瞬时的全光学生物传感技术，以取代ELISA型（酶联免疫吸附测定）测定。利用金属纳米壳的独特光学性质，一种新型的纳米颗粒，含有一个电介质核心涂有一个薄的金属层，免疫测定可以在近红外区域的高生理transmittance（波长在800和1300 nm之间）使用表面增强拉曼散射技术进行。进行这种免疫测定所需的设备将是高度便携和廉价的。对模型抗原的初步研究表明，基于纳米壳的检测可以在20秒内对全血样本产生结果。这在定量上等同于需要24至48小时的ELISA结果。这项研究将探讨将临床上重要的抗体生物结合到纳米壳表面的效果，以及检测用于多抗原/分析物检测的多种拉曼染料。该项目正在开发的技术的主要商业应用是在价值200亿美元的免疫分析市场。 拟议的研究可能导致救护车，军事和民用卫生机构，即时分析和大容量药物测试的快速免疫测定设备。该核心技术可能在生物芯片、基因组学和蛋白质组学研究以及动物生物学领域具有额外的商业应用