Arabidopsis 2010: The power of Arabidopsis in a Halophyte - Tellungiella Halophila (Salt Cress)

拟南芥 2010：拟南芥在盐生植物中的力量 - Tellungiella Halophila（盐水芹）

• 批准号: 0110007
• 负责人: Jian-Kang Zhu
• 金额: $ 12万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0110007&HistoricalAwards=false
• 关键词: Arabidopsis; 2010; power; Halophyte; Tellungiella

High salt in plant growth media causes ion imbalance and hyperosmotic stress. As a consequence of these primary effects, secondary stresses such as oxidative damage often occur. Plant responses to salt stress are very complex, and understanding of salt tolerance mechanisms remains a formidable challenge in agriculture as well as in basic plant biology. Halophytes are naturally salt tolerant plants that have evolved efficient salt tolerance mechanisms. The proposed research aims to dissect salt tolerance mechanisms in halophytes by using the genetically tractable halophilic plant, saltcress (Thellungiella halophila). Several orthologs of Arabidopsis genes involved in salt tolerance will be cloned from saltcress. The cloned genes will be knocked out in saltcress by using the technology of RNA interference. Saltcress mutants thus constructed can be evaluated phenotypically in salt tolerance assays. The proposed studies will help understand how halophytes tolerate salt stress.

植物生长介质中的高盐会引起离子失衡和高渗胁迫。作为这些主要影响的结果，二次应激如氧化损伤经常发生。植物对盐胁迫的反应非常复杂，对耐盐机制的理解在农业和基础植物生物学中仍然是一个巨大的挑战。盐生植物是天然的耐盐植物，进化出了有效的耐盐机制。本研究旨在利用遗传易感的嗜盐植物盐菜（Thellungiella halophila）来剖析盐生植物的耐盐机制。拟南芥耐盐基因的几个同源基因将从盐芥中克隆出来。克隆的基因将通过RNA干扰技术在盐中被敲除。这样构建的盐芥突变体可以在耐盐试验中进行表型评价。拟议的研究将有助于了解盐生植物如何耐受盐胁迫。