Acquisition of a Transmission Electron Microscope to Support UCR's Accelerated Growth in the Cell/Molecular/Development Biology Program

购买透射电子显微镜以支持 UCR 在细胞/分子/发育生物学项目中的加速发展

• 批准号: 0116267
• 负责人: James Baldwin
• 金额: $ 43.59万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0116267&HistoricalAwards=false
• 关键词: Acquisition; Transmission; Electron; Microscope; Support

A grant has been awarded to Dr. James G. Baldwin at The University of California, Riverside (UCR) for a new transmission electron microscope (TEM). The research and teaching needs of this growing campus and scheduling problems on the existing microscope will be met by adding this new TEM. The new microscope is highly complementary with the existing TEM and both instruments will be housed and administered together in the campuswide Central Facility for Advanced Microscopy and Microanalysis (CFAMM) administered within the College of Natural and Agricultural Sciences (CNAGS). This instrument provides functionality needed by experts, yet simplicity appreciated by students and less experienced microscopists. The new microscope will be scheduled for projects that leverage its ease-of-use for diverse TEM work done by many biology researchers and students that do not require high accelerating voltage nor analytical capabilities. Flexibility and scheduling conflicts will be further addressed by equipping the microscope with an electronically controlled rotating stage (required by many Cell, Molecular and Developmental Biology [CMDB]) investigators, e.g. those using stereo pairs) and digital image capture required for most CMDB scientists and crucial for cost effective printing, storage, 3D reconstruction, and easy interfacing with the adjacent Center for Visual Computing (CVC) with which it will be linked by an existing fiber optics system. A new vacuum evaporator is also awarded and is vital for sample preparation for the electron microscope. UCR is the most rapidly growing UC campus, with the highest representation of minority students in the UC system and among the highest nationally. New faculty hires and new laboratory construction are underway to accommodate a 100% increase by 2010, with particular growth in life sciences. The new TEM is essential for research and teaching programs to keep pace with the rapidly expanding interdepartmental program in CMDB and to address a worsening scheduling crisis on the only existing modern TEM on campus and shared by both life and materials scientists. For a significant group of Federally-funded UCR researchers in the life sciences, TEM is an indispensable tool, complementary to a range of additional techniques for acquiring structural information at the cellular and sub-cellular level. These include special applications in cell biology that are relevant to basic science as well as agriculture, biomedicine, pathogenesis, mode of action of antibiotics, and exploring character changes in evolution. UCR's existing CFAMM, interfaced with the CVC, provides a unique context for the new TEM dedicated for life science/CMDB research and training. The new TEM will be the primary graduate and undergraduate TEM teaching tool for seven existing courses, and the PIs of this proposal will collaborate to develop a new training course specific to TEM techniques centered around the new instrument at the CFAMM.

加州大学河滨分校(UCR)的詹姆斯·G·鲍德温(James G.Baldwin)博士获得了一项新的透射式电子显微镜(TEM)的资助。增加这个新的电子显微镜，将满足这个不断发展的校区的研究和教学需求，以及现有显微镜上的调度问题。新的显微镜与现有的电子显微镜是高度互补的，这两种仪器将被安置在校园范围内的高级显微镜和显微分析中央设施(CFAMM)中并一起管理，该中心设在自然和农业科学院(CNAGS)内。这种仪器提供了专家所需的功能，但简单却受到学生和经验较少的显微镜专家的欢迎。这种新型显微镜将用于这样的项目，这些项目利用其易用性，由许多生物研究人员和学生完成不需要高加速电压或分析能力的各种透射电子显微镜工作。灵活性和调度冲突将通过为显微镜配备电子控制的旋转台(许多细胞、分子和发育生物学[CMDB]所需的)和数字图像捕获来进一步解决(许多细胞、分子和发育生物学[CMDB]的研究人员，例如使用立体对的研究人员)和数字图像捕获，这是大多数CMDB科学家所需的，并且对于经济高效的打印、存储、3D重建以及与相邻的视觉计算中心(CVC)的轻松接口至关重要，CVC将通过现有的光纤系统连接到该中心。一种新的真空蒸发器也被授予，这对电子显微镜的样品制备至关重要。UCR是发展最快的加州大学校园，在加州大学系统中少数民族学生的比例最高，在全国名列前茅。新的教员招聘和新的实验室建设正在进行中，以适应到2010年100%的增长，特别是生命科学领域的增长。新的TEM对于研究和教学项目至关重要，以跟上CMDB迅速扩大的跨部门项目的步伐，并解决校园内唯一存在的、由生命和材料科学家共享的现代TEM日益恶化的日程危机。对于一大批由联邦政府资助的UCR生命科学研究人员来说，瞬变电磁是一种不可或缺的工具，是对在细胞和亚细胞水平上获取结构信息的一系列附加技术的补充。这些包括与基础科学以及农业、生物医学、发病机制、抗生素的作用方式以及探索进化过程中的特征变化有关的细胞生物学的特殊应用。UCR现有的CFAMM与CVC接口，为致力于生命科学/CMDB研究和培训的新的TM提供了独特的背景。新的瞬变电磁法将是现有七门课程的主要研究生和本科瞬变电磁学教学工具，该计划的PI将合作开发一门专门针对CFAMM新仪器的瞬变电磁法技术的新培训课程。