MRI: Acquisition of a Transmission Electron Microscope and Digital Imaging: Research and Research Training in an Undergraduate College Environment

已向纽约州立大学购买学院的Jan Robert Factor博士提供赠款，以购买数字透射电子显微镜和有关设备，用于数字图像处理和显微图像分析。具体而言，将获得TEM;将升级图像分析系统;以及用于制备TEM生物样品的超薄切片机和金刚石刀。TEM和相关仪器将用于在本科大学环境中开展多个研究项目和研究培训。该设备将用于龙虾（Homarus americanus）细胞免疫防御领域的研究项目;龙虾（Homarus americanus）的消化组织; Renibacterium salmoninarum细胞表面的表征; Renibacterium salmoninarum在鳟鱼巨噬细胞中的吸收和生长;脂肪酸诱导的细胞事件。1.细胞免疫防御在龙虾，美洲Homarus，扬罗伯特因子。将使用荧光显微镜和数字图像分析研究通过大量固定吞噬细胞从龙虾血液中去除外来颗粒，以确定不利的环境条件和人为毒素是否会导致疾病和大规模死亡。TEM将用于阐明固定吞噬细胞吞噬摄取的不寻常机制。2.龙虾的消化组织，Homarus americanus，Jan Robert Factor。在这个生态和经济上重要的物种的消化过程的理解一直依赖于电子显微镜观察，并在了解消化组织的进一步进展绝对需要使用透射电子显微镜。研究将检查被膜腺的细胞和组织，复杂的基底膜，结缔组织纤维和组织结构。3.鲑肾杆菌细胞表面的表征：菌毛和p57表面蛋白，James G.戴利R.鲑弧菌是引起鲑科鱼类细菌性肾病的一种独特的细胞内病原体。其主要细胞表面抗原（p57）可能对发病机制很重要。p57和表面菌毛可能相同，但p57抗体不能附着于菌毛。透射电子显微镜将用于检查细胞表面，以确定p57是否确实是菌毛或它们是否是独特的蛋白质。4.鲑鱼肾杆菌在鳟鱼巨噬细胞中的摄取和生长。戴利 本项目将利用体外和体内感染的巨噬细胞来跟踪R.鲑鱼巨噬细胞内的沙门氏菌5.脂肪酸诱导的细胞事件的超微结构定位。将通过TEM定位生长因子和类花生酸信号传导的生化途径，以确定特定膳食脂肪酸施用对细胞增殖的影响，以及脂肪酸对程序性细胞死亡的影响。结合细胞培养和生物化学研究，这将进一步加深我们对外源性因素在细胞增殖中作用的理解。这些设备将大大提高采购学院的研究能力，并使教师的研究，这是现在不可能的，对超过一半的生物学教师的研究产生重要影响。该研究包括几个具有实际科学意义的领域：两个商业上重要的动物群体（美国龙虾和鲑鱼）的疾病防御和过程，以及膳食脂肪酸对细胞增殖和程序性细胞死亡的影响。本科生将在两门课程中接受所需技术的培训，提供参与教师研究项目所需的工具。所要求的设备还将用于鼓励学生通过各种当地学校项目追求科学事业。

A grant has been awarded to Dr. Jan Robert Factor at Purchase College, State University of New York, to acquire a digital transmission electron microscope (TEM) and related equipment for digital image processing and microscopical image analysis. Specifically, a TEM will be acquired; an image analysis system will be upgraded; as well as an ultramicrotome and diamond knife for preparing biological samples for TEM. The TEM and related instruments will be used to carry out several research projects and research training in an undergraduate college environment. This equipment will enable research projects in the areas of cellular immune defenses in the lobster (Homarus americanus); digestive tissues of the lobster (Homarus americanus); characterization of the cell surface of Renibacterium salmoninarum; uptake and growth of Renibacterium salmoninarum in trout macrophage; cellular events induced by fatty acids.The following research projects will be carried out. 1. Cellular Immune Defenses in the Lobster, Homarus americanus, Jan Robert Factor. Removal of foreign particles from the blood of the lobster by a large population of fixed phagocytes will be studied using fluorescence light microscopy and analysis of digital images, to determine if adverse environmental conditions and anthropogenic toxins cause disease and mass mortalities. TEM will be used to elucidate the unusual mechanism of phagocytic uptake by the fixed phagocytes. 2. Digestive Tissues of the Lobster, Homarus americanus, Jan Robert Factor. Understanding of digestive processes in this ecologically and economically important species has been reliant upon electron-microscopical observations, and further progress in understanding digestive tissues absolutely requires the use of transmission electron microscopy. Studies will examine cells and tissues of the tegumental glands, complex basement membranes, connective-tissue fibers, and tissue organization. 3. Characterization of the Cell Surface of Renibacterium salmoninarum: Fimbriae and p57 Surface Proteins, James G. Daly. R. salmoninarum is a unique intracellular pathogen that causes bacterial kidney disease in salmonid fishes. Its major cell surface antigen (p57) may be important to pathogenesis. p57 and surface fimbriae may be the same, however, p57 antibody fails to attach to the fimbriae. Transmission electron microscopy will be used to examine the cell surface to determine whether p57 is indeed a fimbria or whether they are distinctive proteins. 4. Uptake and Growth of Renibacterium salmoninarum in Trout Macrophage, James G. Daly. This project will use both in vitro and in vivo infected macrophages to follow the infection and survival of R. salmoninarum within trout macrophage. 5. Ultrastructural Localization of Cellular Events Induced by Fatty Acids, Joanne Kivela Tillotson. Biochemical pathways of growth factor and eicosanoid signaling will be localized by TEM in order to define the influence of specific dietary fatty acid administration upon cell proliferation, and the effects of the fatty acids on programmed cell death. Combined with cell culture and biochemical studies, this will further our understanding of the role of exogenous factors in cell proliferation. This equipment will significantly enhance the research capabilities at Purchase College and enable faculty research that is not now possible, with an important impact on the research of more than half of the Biology faculty. The research includes several areas of practical scientific significance: disease defenses and processes in two commercially important groups of animals (the American lobster and salmonid fishes), and the influence of dietary fatty acids on cell proliferation and programmed cell death. Undergraduate students will be trained in the required techniques in two courses, providing the tools needed to participate in the faculty research projects. The requested equipment will also be used to encourage students to pursue science careers through a variety of local school programs.