ME: An Integrated Metabolic Engineering Study of Evolved Alcohol Acetyl Transferase Enzymes in Flavor Compound Formation in E. coli

ME：进化醇乙酰转移酶在大肠杆菌风味化合物形成中的综合代谢工程研究

• 批准号: 0118815
• 负责人: Ka-yiu San
• 金额: --
• 依托单位: William Marsh Rice University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-11-01 至 2005-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0118815&HistoricalAwards=false
• 关键词: ME; Integrated; Metabolic; Engineering; Study

The objective of the proposed research is to study the microbial production of flavor compounds based on metabolic and genetic engineering. The primary objective is to investigate the response of the central metabolic pathway in Escherichia coli in the presence of perturbations in the cofactor CoA. The specific goals of the research are to: (1) study the redistribution of metabolic fluxes in E. coli with alteration of CoA metabolism by the use of mutant strains with perturbations either in the synthesis or in the usage pathways of the cofactor CoA, (2) examine the effect of these genetic manipulations on the cellular capacity to produce flavor compounds under defined bioreactor experiments, and (3) perform directed evolution of the alcohol acetyltransferase to alter its specificity to make a different pattern of esters. The framework developed in this research could be applied to the improvement of metabolic function for bioprocess applications related to biobased industrial products.

该研究的目的是研究基于代谢和基因工程的风味化合物的微生物生产。 主要目的是研究在辅因子CoA存在扰动的情况下，大肠杆菌中的中心代谢途径的反应。 本研究的具体目标是：（1）研究大肠杆菌代谢流的再分配;通过使用在辅因子CoA的合成或使用途径中具有扰动的突变菌株来改变CoA代谢的大肠杆菌，（2）在限定的生物反应器实验下检查这些遗传操作对细胞产生风味化合物的能力的影响，和（3）进行醇乙酰转移酶的定向进化以改变其特异性以产生不同模式的酯。 在这项研究中开发的框架可以应用于生物基工业产品相关的生物过程应用的代谢功能的改善。