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Enzymology of the bacterial cell wall recycling

细菌细胞壁回收的酶学

基本信息

批准号：
15275545
负责人：
Professor Dr. Christoph Mayer
金额：
--
依托单位：
Lehrstuhl Organismische Interaktionen
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2006
资助国家：
德国
起止时间：
2005-12-31 至 2014-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/15275545?language=en
关键词：
Enzymology bacterial cell wall recycling

项目摘要

An intrinsic consequence of bacterial growth is the breakdown of the expanding cell wall and the release of peptidoglycan turnover products that can be reutilized (recycled). Little is known about cell wall recycling except for the Gram-negative bacterium Escherichia coli. However, Gram-positive bacteria generate peptidoglycan turnover products different from those of Gram-negative bacteria and recycling very likely proceeds through different pathways. Knowing the differences and the enzymology behind these processes would allow new selective strategies for antibiotic treatment of Gram-positive and Gram-negative bacteria. We want to study peptidoglycan recycling in the Gram-positive model organism Bacillus subtilis. The enzymology of two classes of enzymes involved in peptidoglycan recycling will be studied in detail. First, we will be studying the N-acetylglucosaminidases to establish the mechanistical consequences of these enzymes differing in size, domain structure and localization as well as acting on different substrates in Gram-positive and Gram-negative bacteria. Second, we will be studying the lactyl etherases which cleave the D-lactyl ether substituent of the cell wall sugar N-acetylmuramic acid and which were identified in our group recently. They represent a novel class of etherases that presumably catalyze a ß-elimination (lyase-typ) reaction.

细菌生长的一个内在后果是破坏扩张的细胞壁，释放可再利用的肽聚糖周转产物。除了革兰氏阴性杆菌大肠杆菌外，人们对细胞壁的再循环知之甚少。然而，革兰氏阳性菌产生的肽聚糖转化产物与革兰氏阴性菌不同，回收很可能通过不同的途径进行。了解这些过程背后的差异和酶学将为革兰氏阳性和革兰氏阴性细菌的抗生素治疗提供新的选择策略。我们希望研究革兰氏阳性模式生物枯草芽孢杆菌的肽聚糖循环。对参与肽聚糖循环的两类酶的酶学进行了详细的研究。首先，我们将研究n -乙酰氨基葡萄糖酶，以确定这些酶在大小、结构域结构和定位上的不同以及在革兰氏阳性和革兰氏阴性细菌中作用于不同底物的机制后果。其次，我们将研究我们小组最近发现的能够裂解细胞壁糖n -乙酰氨基乙酸的d -丙基醚取代基的乙酰醚酶。它们代表了一类新的醚酶，可能催化ß-消除（裂解酶型）反应。