Laboratory Investigations Using Quantitative Microscopy

生物科学(61)这个项目通过将数字技术引入课堂，将定量方法完全整合到脊椎动物显微解剖学课程中。虽然数字成像技术现在广泛可用，但在本科课程中有效地使用它是有限的，因为缺乏现成的方案、实验室材料和本科显微镜实验练习。此外，对小组的软件指导通常在远离生物实验室的计算机实验室进行。为了解决这些问题，我们正在1)开发一份特别强调图像处理和显微镜定量方法的实验室手册，2)创建应用这些协议的具有生物学意义的调查，学生们从其中收集数字光和电子显微照片，3)使用学生生成的图像以及商业上可用的数据集，使用现成的软件(Adobe Photoshop和IPTK插件)教授图像处理和定量分析，以及4)在生物实验室的工作台上使用无线联网iBook计算机教授图像处理和分析。到课程结束时，学生应该能够准备用于光学和电子显微镜的脊椎动物组织，捕捉数字图像，识别和描述特征组织结构，并在样本之间进行定量比较。

Biological Sciences (61)This project is fully integrating quantitative methods into a course in vertebrate microanatomy by adapting digital technology into the classroom. While digital imaging technology is now widely available, using it effectively in undergraduate courses is limited by a lack of readily available protocols, lab materials, and lab exercises for undergraduate microscopy. In addition, software instruction to groups is usually done in computer labs distant from the biology laboratory. To address these problems, we are 1) developing a laboratory manual with special emphasis on image processing and quantitative methods for microscopy, 2) creating biologically meaningful investigations that apply the protocols, and from which students are collecting digital light and electron micrographs, 3) using the images students generate as well as commercially available datasets to teach image processing and quantitative analysis using readily available software (Adobe Photoshop and IPTK plugins), and 4) teaching image processing and analysis using wireless networked iBook computers at the benches in biology laboratories. By the end of the course, students should be able to prepare vertebrate tissues for light and electron microscopy, capture digital images, identify and describe characteristic tissue structures and make quantitative comparisons between samples.