A Host Protein Interacting With a Tombusvirus Cell-to-Cell Movement Protein

宿主蛋白与汤姆布斯病毒细胞间运动蛋白相互作用

• 批准号: 0131552
• 负责人: Herman Scholthof
• 金额: $ 33万
• 依托单位: Texas A&M Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0131552&HistoricalAwards=false
• 关键词: Host; Protein; Interacting; Tombusvirus; Cell

Viruses are suitable tools to study trafficking of macromolecules through plants because they encode specialized movement proteins (MPs) that enable these pathogens to move cell to cell via the plasmodesmata. Moreover, the MP encoded by the single-stranded positive-sense RNA genome of Tomato bushy stunt virus (TBSV) is functional in many different families. Recent data have shown that the TBSV MP interacts with a novel host factor (HF3.5) with similarities to a class of transcription factors that are known to traffic through plasmodesmata. Results of in vivo and in vitro binding tests with defined MP mutants provided support for the hypothesis that the interaction between HF3.5 and the TBSV MP is functionally relevant. The objectives of this project aim to further test this hypothesis by: I) a detailed molecular analysis of the P22-HF3.5 interaction, II) localization of the HF3.5 protein in planta, III) biochemical verification of P22-host protein interactions in plant extracts, and IV) studying the functional role by monitoring the effect of HF3.5 gene silencing on TBSV movement and infection. It is anticipated that the studies of this newly described movement-associated host protein will provide novel insights into the interactions that control movement of viruses and other macromolecules through plants.

病毒是研究植物大分子运输的合适工具，因为它们编码特殊的运动蛋白（MPs），使这些病原体能够通过胞间连丝在细胞间移动。此外，番茄丛矮病毒（TBSV）单链正义RNA基因组编码的MP在许多不同的科中都有功能。最近的数据表明，TBSV MP与一种新的宿主因子（HF3.5）相互作用，该因子与一类已知通过胞间连丝运输的转录因子相似。确定的MP突变体的体内和体外结合试验结果支持了HF3.5与TBSV MP之间相互作用在功能上相关的假设。本项目的目标是进一步验证这一假设：1)对P22-HF3.5相互作用的详细分子分析，2)HF3.5蛋白在植物中的定位，3)植物提取物中p22 -宿主蛋白相互作用的生化验证，4)通过监测HF3.5基因沉默对TBSV运动和感染的影响来研究其功能作用。预计对这种新描述的运动相关宿主蛋白的研究将为控制病毒和其他大分子通过植物运动的相互作用提供新的见解。