NSF-EC: Biochemistry of Algal Adhesives

该奖项授予加州大学圣巴巴拉分校，目的是从不同藻类中分离和鉴定粘附性糖蛋白，并确定这些蛋白及其亚基的相对分子质量、形状和初级序列。凭借这一奖项，John Waite教授将评估粘着蛋白的化学功能，特别是翻译后修饰的测定，并将这些修饰与贻贝粘着蛋白中观察到的修饰进行比较。将对粘接蛋白进行建模，并研究在粘接固化过程中控制交联反应的方法。将与该项目合作的欧洲科学家将为这些研究提供藻类粘连蛋白、卤代过氧化物酶和cDNA推导的蛋白质序列。这个由六个大学研究团队、两家生物技术公司和欧洲科学家组成的多人项目将分离、表征、模拟和批量生产藻类粘连蛋白，最终目的是开发药用粘合剂并减轻污染。许多分析方法，包括碰撞诱导分解的串联质谱仪，将被用来确定这些蛋白质及其亚基的初级序列。该NSF项目由多学科活动办公室、化学(先进材料)、材料研究(固态化学)、国际办公室(西欧)和土木和机械系统(表面和材料设计)部门共同资助，作为国家科学基金会和欧洲委员会在材料科学方面的合作活动(NSF 01-105)。该项目是与以色列海洋和湖泊研究所、以色列理工学院、英国伯明翰大学、瑞典哥德堡大学、法国罗斯科夫海洋站以及来自冰岛(Bio-Gels制药有限公司)和以色列(Biota有限公司)的工业合作者合作开展的。

This award to University of California Santa Barbara is to isolate and characterize adhesive glycoproteins from different algae species, and to determine molecular weight, shape and primary sequence of these proteins and their subunits. With this award Professor John Waite will evaluate the chemical functionality of the adhesive proteins, especially the determination of post-translational modifications and comparison of these modifications to those observed in adhesive proteins from mussels. Modeling of the adhesive proteins and studying methods to control cross-linking reactions during the adhesive curing process will be carried out. European scientists, who will be collaborating with this project, will provide algal adhesive proteins, haloperoxidase and cDNA-deduced protein sequences for these studies.This multi investigator project with six university research teams, two biotech companies and European scientists will isolate, characterize, mimic, and bulk-produce algal adhesive proteins with the ultimate aim of developing pharmaceutical adhesives and to mitigate fouling. Many analytical methods including tandem mass spectrometry with collision-induced decomposition will be used to determine the primary sequence of these proteins and their subunits. This NSF project is co-funded by the Office of Multidisciplinary Activities, and Divisions of Chemistry (Advanced Materials), Materials Research (Solid State Chemistry), International Office (Western Europe) and Civil and Mechanical Systems (Surface and Material Design) as a Cooperative Activity in Materials Sciences between the National Science Foundation and the European Commission (NSF 01-105). This project is being carried out in collaboration with the Israel Oceanographic and Limnological Research Institute; Technion University, Israel; University of Birmingham, England; Gothenburg University, Sweden; Roscoff Marine Station, France; and industrial collaborators from Iceland (Bio-Gels Pharmaceuticals Ltd) and Israel (Biota Ltd).