NSF-EC: Biochemistry of Algal Adhesives

NSF-EC：藻类粘合剂的生物化学

• 批准号: 0132443
• 负责人: J Herbert Waite
• 金额: $ 36万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0132443&HistoricalAwards=false
• 关键词: NSF; EC; Biochemistry; Algal; Adhesives

This award to University of California Santa Barbara is to isolate and characterize adhesive glycoproteins from different algae species, and to determine molecular weight, shape and primary sequence of these proteins and their subunits. With this award Professor John Waite will evaluate the chemical functionality of the adhesive proteins, especially the determination of post-translational modifications and comparison of these modifications to those observed in adhesive proteins from mussels. Modeling of the adhesive proteins and studying methods to control cross-linking reactions during the adhesive curing process will be carried out. European scientists, who will be collaborating with this project, will provide algal adhesive proteins, haloperoxidase and cDNA-deduced protein sequences for these studies.This multi investigator project with six university research teams, two biotech companies and European scientists will isolate, characterize, mimic, and bulk-produce algal adhesive proteins with the ultimate aim of developing pharmaceutical adhesives and to mitigate fouling. Many analytical methods including tandem mass spectrometry with collision-induced decomposition will be used to determine the primary sequence of these proteins and their subunits. This NSF project is co-funded by the Office of Multidisciplinary Activities, and Divisions of Chemistry (Advanced Materials), Materials Research (Solid State Chemistry), International Office (Western Europe) and Civil and Mechanical Systems (Surface and Material Design) as a Cooperative Activity in Materials Sciences between the National Science Foundation and the European Commission (NSF 01-105). This project is being carried out in collaboration with the Israel Oceanographic and Limnological Research Institute; Technion University, Israel; University of Birmingham, England; Gothenburg University, Sweden; Roscoff Marine Station, France; and industrial collaborators from Iceland (Bio-Gels Pharmaceuticals Ltd) and Israel (Biota Ltd).

该奖项授予加州大学圣巴巴拉是分离和表征粘附糖蛋白从不同的藻类物种，并确定分子量，形状和一级序列的这些蛋白质及其亚基。 有了这个奖项，John Waite教授将评估粘附蛋白的化学功能，特别是翻译后修饰的测定，以及这些修饰与贻贝粘附蛋白中观察到的修饰的比较。 将进行粘附蛋白的建模和研究方法来控制粘合剂固化过程中的交联反应。 欧洲科学家将与该项目合作，为这些研究提供藻类粘附蛋白、卤素过氧化物酶和cDNA推导的蛋白质序列。这个由六个大学研究团队、两家生物技术公司和欧洲科学家组成的多研究者项目将分离、表征、模拟和批量生产藻类粘附蛋白，最终目的是开发药物粘合剂和减轻污垢。许多分析方法，包括串联质谱与碰撞诱导分解将被用来确定这些蛋白质及其亚基的一级序列。该NSF项目由多学科活动办公室、化学（先进材料）、材料研究（固态化学）、国际办公室（西欧）和土木与机械系统（表面和材料设计）部门共同资助，是国家科学基金会和欧洲委员会之间的材料科学合作活动（NSF 01-105）。该项目是与下列机构合作开展的：以色列海洋学和湖沼学研究所、以色列Technion大学、英国伯明翰大学、瑞典哥德堡大学、法国Roscoff海洋站以及冰岛（Bio-Gels制药有限公司）和以色列（Biota有限公司）的工业合作者。