A Multi-User Proposal to Purchase A Scanning Laser Confocal Microscope

该奖项支持购买最先进的扫描激光共聚焦显微镜系统，该系统包括一个带有电动舞台和多个激光器的直立显微镜。该仪器配备了使用需要油或水浸泡的方法，并具有快速扫描能力，适合于研究经历快速生理变化的运动细胞。将使用显微镜的研究项目包括研究精子-卵子的相互作用以及海洋和淡水生物中围绕受精的生理和生化事件。其他项目包括研究环境挑战后与分子伴侣细胞内易位有关的细胞代谢抑制，以及研究热带珊瑚的光漂白及其与紫外线引起的DNA损伤的关系。除了在研究方面的作用外，该仪器还将供本科生和研究生在正式课程和有关训练活动中使用。该仪器将与其他用于补充方法的仪器一起放置在Bodega海洋实验室的荧光成像设施中。该仪器将供大学主校区以及实验室的研究人员和学生使用。

This award supports purchase of a state-of-the-art scanning laser confocal microscopy system that includes an upright microscope with motorized stage and multiple lasers. The instrument is equipped for use of methods requiring oil or water immersion, and has fast scanning capabilities suitable for study of motile cells undergoing rapid physiological changes. Research projects that will employ the microscope include studies of sperm-egg interactions and the physiological and biochemical events surrounding fertilization in marine and freshwater organisms. Other projects include studies of metabolic depression in cells connected with intracellular translocation of a molecular chaperone after environmental challenges, and studies of photobleaching of corals in the tropics and its relationship to DNA damage caused by ultraviolet light. In addition to its role in research, the instrument will be used by undergraduate and graduate students in formal courses and related training activities. The instrument will be located in the Fluorescence Imaging Facility at the Bodega Marine Laboratory along with other instruments used for complementary methods. The instrument will be available for use by investigators and students from the University's main campus as well as those at the Laboratory.