A Multi-User Proposal to Purchase A Scanning Laser Confocal Microscope

购买扫描激光共焦显微镜的多用户提案

• 批准号: 0202723
• 负责人: Gary Cherr
• 金额: $ 20.56万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0202723&HistoricalAwards=false
• 关键词: Multi; User; Proposal; Purchase; Scanning

This award supports purchase of a state-of-the-art scanning laser confocal microscopy system that includes an upright microscope with motorized stage and multiple lasers. The instrument is equipped for use of methods requiring oil or water immersion, and has fast scanning capabilities suitable for study of motile cells undergoing rapid physiological changes. Research projects that will employ the microscope include studies of sperm-egg interactions and the physiological and biochemical events surrounding fertilization in marine and freshwater organisms. Other projects include studies of metabolic depression in cells connected with intracellular translocation of a molecular chaperone after environmental challenges, and studies of photobleaching of corals in the tropics and its relationship to DNA damage caused by ultraviolet light. In addition to its role in research, the instrument will be used by undergraduate and graduate students in formal courses and related training activities. The instrument will be located in the Fluorescence Imaging Facility at the Bodega Marine Laboratory along with other instruments used for complementary methods. The instrument will be available for use by investigators and students from the University's main campus as well as those at the Laboratory.

该奖项支持购买最先进的扫描激光共聚焦显微镜系统，包括一个带电动载物台和多个激光器的立式显微镜。 该仪器配备用于需要油或水浸泡的方法，并具有快速扫描能力，适用于研究经历快速生理变化的运动细胞。 使用该显微镜的研究项目包括研究精卵相互作用以及海洋和淡水生物受精周围的生理和生化事件。 其他项目包括与环境挑战后分子伴侣细胞内移位有关的细胞代谢抑制研究，以及热带珊瑚光漂白及其与紫外线造成的DNA损伤之间关系的研究。 除了在研究中的作用外，本科生和研究生还将在正式课程和相关培训活动中使用该仪器。该仪器将与用于补充方法的其他仪器一起沿着放置在博德加海洋实验室的荧光成像设施中。 该仪器将供大学主校区和实验室的研究人员和学生使用。