International Research Fellowship Program: Origins of Polyploidy in Achillea Millefolium

国际研究奖学金计划：蓍草多倍体的起源

• 批准号: 0202729
• 负责人: Justin Ramsey
• 金额: $ 8.41万
• 依托单位: Ramsey Justin M
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2005-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0202729&HistoricalAwards=false
• 关键词: International; Research; Fellowship; Program; Origins

0202729RamseyThe International Research Fellowship Program enables U.S. scientists and engineers to conduct three to twenty-four months of research abroad. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad.This award will support a twenty-four month research fellowship by Dr. Justin M. Ramsey to work with Dr. Brian C. Husband at the University of Guelph, Ontario, Canada.This project will involve the investigation of Achillea millefolium (asteraceae), a native wildflower with tetraploid and hexaploid populations. Recent studies document the local adaptation of hexaploid genotypes to xeric environments. This research extends those investigations to address the following questions: (1) What are the dynamics of polyploid establishment, and how do genetic and ecological factors influence this process?; and (2) What is the phylogenetic history of polyploidy in North American Achillea? Numerical simulations based on a life-history model (transitions from gamete to seed, seed to seedling, seedling to adult, and adult to gamete) will track the fate of hexaploid mutants in tetraploid populations, and identify the conditions in which hexaploid populations establish and persist. Previous empirical data will be used to parameterize the simulation. Phylogenetic analyses of Amplified Fragment Length Polymorphisms (AFLP) genetic markers and DNA sequence data will be conducted at both regional and continental geographic scales to quantify local incidences of hexaploid formation as well as successful establishments of hexaploids over the species range. This multi-faceted research will elucidate the mechanisms, ecological significance, and patterns of polyploid evolution.Dr. Husband has a strong background in molecular markers, population genetics, and quantitative methods in ecology and evolution. He has developed quantitative approaches in polyploidy research.

0202729拉姆齐国际研究奖学金计划使美国科学家和工程师能够在国外进行三到二十四个月的研究。 该项目的奖项为联合研究以及使用国外独特或互补的设施、专业知识和实验条件提供了机会。该奖项将支持 Justin M. Ramsey 博士与加拿大安大略省圭尔夫大学的 Brian C. Husband 博士合作为期 24 个月的研究奖学金。该项目将涉及对 Achillea millefolium（菊科）的调查，这是一种具有四倍体和 六倍体群体。 最近的研究记录了六倍体基因型对干旱环境的局部适应。 这项研究扩展了这些研究，以解决以下问题：（1）多倍体建立的动态是什么，遗传和生态因素如何影响这一过程？ (2) 北美蓼属多倍体的系统发育史是怎样的？ 基于生活史模型（从配子到种子、种子到幼苗、幼苗到成体、成体到配子的转变）的数值模拟将追踪四倍体种群中六倍体突变体的命运，并确定六倍体种群建立和持续的条件。 先前的经验数据将用于参数化模拟。 扩增片段长度多态性 (AFLP) 遗传标记和 DNA 序列数据的系统发育分析将在区域和大陆地理尺度上进行，以量化六倍体形成的局部发生率以及在物种范围内成功建立六倍体。 这项多方面的研究将阐明多倍体进化的机制、生态意义和模式。丈夫在分子标记、群体遗传学以及生态学和进化方面的定量方法方面拥有深厚的背景。 他开发了多倍体研究的定量方法。