SBIR Phase I: Bioremediation of N-nitrosodimethylamine in Groundwater

SBIR 第一阶段：地下水中 N-亚硝基二甲胺的生物修复

• 批准号: 0212405
• 负责人: Paul Hatzinger
• 金额: $ 10万
• 依托单位: Envirogen, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0212405&HistoricalAwards=false
• 关键词: SBIR; Phase; Bioremediation; nitrosodimethylamine; Groundwater

This Small Business Phase I Project is to develop a bioremediation technology for treating groundwater contaminated with N-nitrosodimethylamine (NDMA). NDMA is a potent carcinogen and an emerging groundwater contaminant in the United States. Accordingly, the State of California has established an acceptable level in drinking water for NDMA of only 20 ng/L. Current technologies for treating NDMA, such as ultraviolet irradiation and carbon adsorption, are expensive and/or ineffective for removing the contaminant to required levels. Envirogen scientists have recently discovered a bacterial strain that is capable of metabolizing NDMA during growth on a second substrate (i.e., by co-metabolism). This bacterium is one of only a few strains that are known to degrade NDMA. In the course of this Phase I project, the biodegradation of NDMA by this organism andby other bacterial strains possessing similar broad specificity oxidase enzymes will be examined. The most effective culture(s) will be seeded into bioreactors and the abilities of these strains to remediate NDMA in groundwater will be quantified. In addition, the potential tostimulate specific microorganisms in contaminated aquifers to degrade NDMA by cosubstrate application will be tested. The commercial application of this project is in the area of wastewater treatment linked to municipal drinking water supplies. Additional industrial and military uses of the core technology are also expected.

该小型企业一期项目旨在开发一种生物修复技术，用于处理受N-亚硝基二甲胺（NDMA）污染的地下水。NDMA是一种强效致癌物，也是美国新兴的地下水污染物。因此，加州州已确定饮用水中NDMA的可接受水平仅为20 ng/L。目前处理NDMA的技术，如紫外线照射和碳吸附， 对于将污染物去除到所需水平来说是昂贵的和/或无效的。Envirogen的科学家最近发现了一种细菌菌株，该菌株能够在第二底物上生长期间代谢NDMA（即，共代谢）。这种细菌是已知能降解NDMA的少数菌株之一。在第一阶段的项目中，将检测该微生物和其他具有类似广泛特异性氧化酶的细菌菌株对NDMA的生物降解。将最有效的培养物接种到生物反应器中，并量化这些菌株修复地下水中NDMA的能力。此外，还将测试通过共基质应用刺激受污染含水层中特定微生物降解NDMA的潜力。 该项目的商业应用是与城市饮用水供应有关的废水处理领域。预计该核心技术还将用于其他工业和军事用途。