Identification and Characterization of Cell Wall Mutants in Maize and Arabidipsis using Novel Spectroscopies

傅里叶变换中程光谱和近红外光谱判别分析两种方法已经发展成为鉴定植物细胞壁多糖成分和细胞壁结构突变的高通量方法。这些方法将用于在插入标记的玉米和拟南芥群体中识别与生长发育过程中植物细胞壁结构的生物发生和动态改变有关的广泛突变体。采用生化、光谱学和成像方法的系统方案已被设计出来，将壁结构和结构中的缺陷分类为与壁生物发生或解体的六个阶段之一。携带mu插入的玉米系和携带T-DNA标签的拟南芥系将作为正向和反向筛选的主要材料。该项目的一个主要实际目标是产生具有遗传定义的壁面组成和结构变异的植物，从而可以评估壁面特性和植物发育的修改。由于细胞壁是极其重要的工业原料来源，我们预计，我们鉴定和鉴定的一些基因，以及一些具有遗传变异的植物，将具有重要的经济意义。随着突变的遗传性得到确认，以及所鉴定基因的可能功能信息的获得，植物生物学社区将通过一个网站了解这些突变，该网站已被创建为所有细胞壁相关基因组学的存储库。玉米和拟南芥库存中心将提供所有材料分发给整个社区。

Two methods, discriminant analysis of Fourier transform mid-range and near infrared spectra, have been developed as high throughput approaches to identifying mutations in plant cell wall polysaccharide components and wall architecture. These methods will be employed to identify in insertion tagged populations of maize and Arabidopsis a broad range of mutants involved in the biogenesis and dynamic alteration of plant cell wall architecture during growth and development. A systematic protocol using biochemical, spectroscopic, and imaging methods has been devised to categorize defects in wall structure and architecture related to development into one of six stages of wall biogenesis or disassembly. Maize lines carrying Mu-insertions and Arabidopsis lines carrying T-DNA tags will be used as primary material for both forward and reverse screens. A major practical goal of the project is to generate plants with genetically defined variation in wall composition and architecture that will permit assessment of modifications on wall properties and plant development. Since cell walls are an enormously important source of industrial raw material, we anticipate that several of the genes we identify and characterize, as well as several of the plants with genetically defined alterations, will be of economic importance. As the heritability of the mutations is confirmed and information on the possible function of the genes identified is obtained, the plant biology community will be informed of them through a web site that has been created as a repository for all cell-wall related genomics. Maize and Arabidopsis stock centers will be provided with all materials to dispense to the community at large.