Identification and Characterization of Cell Wall Mutants in Maize and Arabidipsis using Novel Spectroscopies

两种方法，判别分析的傅立叶变换中范围和近红外光谱，已被开发为高通量的方法来识别突变的植物细胞壁多糖组分和壁结构。 这些方法将被用来鉴定插入标记的玉米和拟南芥群体中的广泛的突变体，所述突变体参与生长和发育期间植物细胞壁结构的生物发生和动态改变。一个系统的协议，使用生物化学，光谱和成像方法已被设计成分类缺陷壁结构和建筑相关的发展到六个阶段之一的壁生源或拆卸。 携带Mu-插入的玉米系和携带T-DNA标签的拟南芥系将用作正向和反向筛选的主要材料。 该项目的一个主要实际目标是生成具有遗传定义的墙壁组成和结构变化的植物，这将允许评估墙壁特性和植物发育的修改。 由于细胞壁是工业原材料的一个非常重要的来源，我们预计我们鉴定和表征的几个基因，以及几个具有遗传定义改变的植物，将具有经济重要性。随着突变的遗传性得到证实，并获得有关所鉴定基因的可能功能的信息，植物生物学界将通过一个网站了解这些信息，该网站已被创建为所有细胞壁相关基因组学的知识库。 玉米和拟南芥储备中心将提供所有材料分发给整个社区。

Two methods, discriminant analysis of Fourier transform mid-range and near infrared spectra, have been developed as high throughput approaches to identifying mutations in plant cell wall polysaccharide components and wall architecture. These methods will be employed to identify in insertion tagged populations of maize and Arabidopsis a broad range of mutants involved in the biogenesis and dynamic alteration of plant cell wall architecture during growth and development. A systematic protocol using biochemical, spectroscopic, and imaging methods has been devised to categorize defects in wall structure and architecture related to development into one of six stages of wall biogenesis or disassembly. Maize lines carrying Mu-insertions and Arabidopsis lines carrying T-DNA tags will be used as primary material for both forward and reverse screens. A major practical goal of the project is to generate plants with genetically defined variation in wall composition and architecture that will permit assessment of modifications on wall properties and plant development. Since cell walls are an enormously important source of industrial raw material, we anticipate that several of the genes we identify and characterize, as well as several of the plants with genetically defined alterations, will be of economic importance. As the heritability of the mutations is confirmed and information on the possible function of the genes identified is obtained, the plant biology community will be informed of them through a web site that has been created as a repository for all cell-wall related genomics. Maize and Arabidopsis stock centers will be provided with all materials to dispense to the community at large.