Identification and Characterization of Cell Wall Mutants in Maize and Arabidipsis using Novel Spectroscopies

两种方法，傅里叶变换中程和近红外光谱的判别分析，已经发展成为高通量鉴定植物细胞壁多糖组分和壁结构突变的方法。这些方法将被用来在插入标记的玉米和拟南芥群体中鉴定广泛的突变体，这些突变体参与了植物生长发育过程中细胞壁结构的生物发生和动态变化。利用生化、光谱和成像方法设计了一种系统的方案，将与发育相关的墙结构和建筑中的缺陷分类为墙生物发生或拆解的六个阶段之一。携带Mu-插入片段的玉米品系和携带T-DNA标签的拟南芥品系将被用作正反向筛选的主要材料。该项目的一个主要实际目标是培育出在墙体成分和建筑上具有遗传差异的植物，以便评估对墙体特性和植物发展的修改。由于细胞壁是工业原料的一个极其重要的来源，我们预计，我们识别和表征的几个基因，以及几个具有基因定义变化的植物，将具有重要的经济意义。随着突变的遗传性得到确认并获得有关已确定基因可能功能的信息，植物生物界将通过一个网站通知这些突变，该网站是作为所有与细胞壁相关的基因组学的储存库而创建的。将向玉米和拟南芥库存中心提供所有材料，以分发给广大社区。

Two methods, discriminant analysis of Fourier transform mid-range and near infrared spectra, have been developed as high throughput approaches to identifying mutations in plant cell wall polysaccharide components and wall architecture. These methods will be employed to identify in insertion tagged populations of maize and Arabidopsis a broad range of mutants involved in the biogenesis and dynamic alteration of plant cell wall architecture during growth and development. A systematic protocol using biochemical, spectroscopic, and imaging methods has been devised to categorize defects in wall structure and architecture related to development into one of six stages of wall biogenesis or disassembly. Maize lines carrying Mu-insertions and Arabidopsis lines carrying T-DNA tags will be used as primary material for both forward and reverse screens. A major practical goal of the project is to generate plants with genetically defined variation in wall composition and architecture that will permit assessment of modifications on wall properties and plant development. Since cell walls are an enormously important source of industrial raw material, we anticipate that several of the genes we identify and characterize, as well as several of the plants with genetically defined alterations, will be of economic importance. As the heritability of the mutations is confirmed and information on the possible function of the genes identified is obtained, the plant biology community will be informed of them through a web site that has been created as a repository for all cell-wall related genomics. Maize and Arabidopsis stock centers will be provided with all materials to dispense to the community at large.