BE/GEN-EN: Genome Sequence and Functional Analysis of an Uncultured Bacterium

BE/GEN-EN：未培养细菌的基因组序列和功能分析

• 批准号: 0221798
• 负责人: Steven Gill
• 金额: --
• 依托单位: Institute for Genomic Research
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-10-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0221798&HistoricalAwards=false
• 关键词: GEN; EN; Genome; Sequence; Functional

ABSTRACTAt 80 by 600 microns, Epulopiscium is the largest heterotrophic bacterium that has been identified to date. It also has the unusual ability to produce multiple live offspring internally. This project takes an aggressive approach to increase our understanding of the biology of this organism by determining the complete genome sequence, and identifying the unique features of Epulopiscium that have allowed these exceptional bacteria to attain cellular sizes that are orders of magnitude larger than most bacteria. These analyses will identify genomic and cellular modifications that may have been employed in the progenitor of all eukaryotes as a first step toward the development of the contemporary eukaryotic cell. Comparative analyses with the complete genome sequences of endospore-forming bacteria will identify sporulation gene homologues in the Epulopiscium genome that can serve as targets of future analyses to determine the role of these genes in intracellular offspring production. Finally, this project will generate a physiological profile of Epulopiscium sp. based on the complete genome sequence. This component will address how the normal environment of this species (the guts of surgeonfish) may have enabled the evolution of these life-style novelties, and serve as a framework for developing culture media to grow Epulopiscium in the laboratory so that more detailed experiments on this species can be conducted. Collaborations, discussions, and data exchange with both domestic and international groups that are studying Epulopiscium biology, spore-forming bacteria, and extremely large bacteria will be encouraged. Training and educational workshops will be held at The Institute for Genomic Research (TIGR) in Rockville, Maryland for these biologists. The project team will make every attempt to ensure that underrepresented groups are included in the students and post-doctoral candidates who will be involved for the duration of the proposed study. Sequence and microarray data will be continuously posted and updated on our public web site (www.tigr.org).

直径为80 × 600微米的Epulopiscium是迄今为止发现的最大的异养细菌。它也有不寻常的能力，产生多个活的后代内部。该项目采取了积极的方法，通过确定完整的基因组序列，并确定Epulopiscium的独特特征来增加我们对这种生物体生物学的理解，这些独特的特征使这些特殊的细菌能够获得比大多数细菌大几个数量级的细胞大小。这些分析将鉴定可能已经在所有真核生物的祖细胞中采用的基因组和细胞修饰，作为当代真核细胞发育的第一步。与完整的基因组序列的内孢子形成细菌的比较分析将确定孢子形成基因的同源物在Epulopiscium基因组中，可以作为未来的分析，以确定这些基因在细胞内后代生产的作用的目标。最后，该项目将根据完整的基因组序列生成Epulopiscium sp.的生理概况。这一部分将解决这个物种的正常环境（外科鱼的内脏）如何使这些生活方式的新奇的进化，并作为一个框架，用于开发培养基，在实验室中生长Epulopiscium，以便对这个物种进行更详细的实验。将鼓励与正在研究Epulopiscium生物学，孢子形成细菌和超大型细菌的国内和国际团体进行合作，讨论和数据交换。培训和教育讲习班将在马里兰州罗克维尔的基因组研究所（TIGR）为这些生物学家举行。项目小组将尽一切努力确保在拟议研究期间参与的学生和博士后候选人中包括代表性不足的群体。序列和微阵列数据将在我们的公共网站（www.tigr.org）上持续发布和更新。