BE/GEN-EN: Who's Doing What in a Complex Bioreactor? Stable Isotope Probing of Specific Degraders in Engineered Biological Treatment Processes

BE/GEN-EN：谁在复杂的生物反应器中做什么？

• 批准号: 0221836
• 负责人: Michael Aitken
• 金额: $ 56.4万
• 依托单位: University of North Carolina at Chapel Hill
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0221836&HistoricalAwards=false
• 关键词: GEN; EN; Who; Doing; What

0221836 Aitken A stable isotope method will be used to prepare heavy DNA from incubations of mixed cultures and specific C-13 labelled PAH substrates in completely closed slurry aerobic bioreactors. The bioreactor is fitted with a gas chromatograph which allows on- line respirometric measurements. DNA and rRNA will be extracted from the slurry, amplified by PCR, and analyzed on agarose gels. Comparisons between the DGGE profiles obtainedfrom rDNA (PCR), rRNA (RT-PCR) and C-13 labelled rDNA and rRNA will provide insight into the metabolically active members of the community. Selected DGGE bands will be sequenced and submitted for phylogenetic analysis using PHYLIP software.

0221836 Aitken将使用稳定同位素方法，在完全封闭的浆液好氧生物反应器中，通过混合培养物和特定C-13标记的PAH底物的孵育制备重链DNA。 生物反应器配有气相色谱仪，可进行在线呼吸测量。 将从浆液中提取DNA和rRNA，通过PCR扩增，并在琼脂糖凝胶上分析。 比较rDNA（PCR）、rRNA（RT-PCR）和C-13标记的rDNA和rRNA的DGGE图谱，可以了解群落中代谢活跃的成员。 对选定的DGGE条带进行测序，并提交使用PHYLIP软件进行系统发育分析。