BE/GEN-EN: Who's Doing What in a Complex Bioreactor? Stable Isotope Probing of Specific Degraders in Engineered Biological Treatment Processes

0221836 Aitken A稳定同位素方法将用于在完全封闭的泥浆好氧生物反应器中从混合培养物和特定的C-13标记的多环芳烃底物的培养中制备重DNA。该生物反应器配备了一台气相色谱仪，可以进行在线呼吸测量。DNA和rRNA将从浆液中提取，经PCR扩增，并在琼脂糖凝胶上进行分析。通过比较rDNA(聚合酶链式反应)、rRNA(逆转录聚合酶链式反应)和C-13标记的rDNA和rRNA获得的DGGE图谱，将有助于深入了解群落中代谢活跃的成员。选定的DGGE条带将被测序，并提交使用PHYLIP软件进行系统发育分析。

0221836 Aitken A stable isotope method will be used to prepare heavy DNA from incubations of mixed cultures and specific C-13 labelled PAH substrates in completely closed slurry aerobic bioreactors. The bioreactor is fitted with a gas chromatograph which allows on- line respirometric measurements. DNA and rRNA will be extracted from the slurry, amplified by PCR, and analyzed on agarose gels. Comparisons between the DGGE profiles obtainedfrom rDNA (PCR), rRNA (RT-PCR) and C-13 labelled rDNA and rRNA will provide insight into the metabolically active members of the community. Selected DGGE bands will be sequenced and submitted for phylogenetic analysis using PHYLIP software.