QSB: Quantitative Systems Approach for Understanding Hepatic Metabolism

该项目的总体目标是将定量分析与生物学相结合，为了解肝细胞(肝脏)代谢提供一个系统的方法，以获得对II型糖尿病的洞察。该项目的具体目标是：(1)借助代谢通量分析表征环境因素诱导的肝细胞中一系列全面的代谢变化，(2)结合生理化学信息，即动力学结合数据，以提供对肝脏代谢的机械洞察，而不是仅通过代谢通量分析获得的信息，以及(3)利用多变量分析建立现象学模型，以确定对糖尿病表型贡献最大的候选途径或因素。我们的目标是开发一种能够识别糖尿病潜在风险的模型。

The overall goal of this project is to integrate quantitative analysis with biology to provide a systems approach to understanding hepatocyte (liver) metabolism in order to gain insights into Type II diabetes. The specific goals of the project are to: (1) characterize a comprehensive set of metabolic alterations in the hepatocytes induced by environmental factors with the aid of metabolic flux analysis, (2) incorporate physiochemical information; namely, kinetic binding data to provide a mechanistic insight into hepatic metabolism beyond that obtained with metabolic flux analysis alone, and (3) develop a phenomenological model using multivariate analysis to identify candidate pathways or factors that contribute most to the diabetic phenotype. The objective is the development of a model capable of identifying the potential for diabetes.