Training Underrepresented Basic Science Researchers Utilizing Structure-Function Investigations of Neuropeptide Receptors

利用神经肽受体的结构功能研究来培训代表性不足的基础科学研究人员

• 批准号: 0235146
• 负责人: James Porter
• 金额: $ 39.53万
• 依托单位: University of North Dakota Main Campus
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0235146&HistoricalAwards=false
• 关键词: Training; Underrepresented; Basic; Science; Researchers

Principal Investigator: James E. PorterProposal #: 0235146Title of Proposal: Training Underrepresented Basic Science Researchers Utilizing Structure-Function Investigations of Neuropeptide ReceptorsAbstract: The immediate goal of this project is to promote the teaching and training of students by characterizing fundamental processes by which a stimulus is detected and transmitted by organisms to initiate essential biological events. The calcitonin gene-related peptide (CGRP) is an established neurotransmitter that is prominently associated with initiating complex multicellular interactions that regulate or modulate the function of many living organisms (e.g., sensory pathways). Unfortunately, the types of CGRP ligands currently available limit information about the cellular effects associated with CGRP modulation of physiological responses. This proposal will test the hypothesis that insights into the cellular mechanisms of CGRP mediated effects will provide a background for the rational development of novel non-peptide ligands. Pharmacological analysis of CGRP signaling mechanisms for mutant and wild type proteins will be examined using biochemical, molecular and cell biological techniques. Differences in the properties of these mutant proteins from the wild type will be used to advance a molecular model associated with CGRP cellular effects. Furthermore, this knowledge could be used to develop compounds that would help researchers understand the specific modulatory functions associated with this neuropeptide. Moreover, results generated from completion of this proposal will forward our understanding of how ubiquitous peptides are recognized and communicated through sensory pathways using intracellular signaling systems. Finally, this research would benefit the education and training of underrepresented groups in a demographically disadvantaged state, by providing support for advanced training in the biological sciences.

提案编号：0235146提案标题：利用神经肽受体的结构-功能研究培养未被充分代表的基础科学研究人员摘要：本项目的直接目标是通过描述刺激被生物体检测和传递以启动基本生物事件的基本过程来促进学生的教学和培训。降钙素基因相关肽（CGRP）是一种已知的神经递质，与启动复杂的多细胞相互作用密切相关，这些相互作用调节许多生物体的功能（例如，感觉通路）。不幸的是，目前可用的CGRP配体类型限制了与CGRP调节生理反应相关的细胞效应的信息。这一提议将验证这样一个假设，即深入了解CGRP介导的细胞机制将为新型非肽配体的合理开发提供背景。突变型和野生型蛋白的CGRP信号机制的药理学分析将使用生化，分子和细胞生物学技术进行检查。这些野生型突变蛋白的特性差异将用于推进与CGRP细胞效应相关的分子模型。此外，这些知识可以用来开发化合物，帮助研究人员了解与这种神经肽相关的特定调节功能。此外，完成本提案所产生的结果将有助于我们理解如何通过细胞内信号系统的感觉途径识别和交流无处不在的肽。最后，这项研究将通过为生物科学的高级培训提供支持，有利于在人口上处于不利地位的国家中代表性不足的群体的教育和培训。