Microbial Observatories: Eukaryotic Microbial Communities of the Old Woman Creek National Estuarine Research Reserve

微生物观测站：老妇溪国家河口研究保护区的真核微生物群落

• 批准号: 0239997
• 负责人: Peter Lavrentyev
• 金额: $ 22.06万
• 依托单位: University of Akron
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0239997&HistoricalAwards=false
• 关键词: Microbial; Observatories; Eukaryotic; Communities; Old

A grant has been awarded to Drs. Peter Lavrentyev and Robert Duff of the University ofAkron to examine taxonomic composition of planktonic protists in relation to their key functionsat the NOAA Old Woman Creek National Estuarine Research Reserve (OWC NERR). Sampleswill be collected from four stations along the stream-Lake Erie transect at monthly intervals inconjunction with the ongoing long-term monitoring. Microbial community structure,distribution, and dynamics will be examined under different hydrologic and climatic conditionsusing molecular, digital video, flow-cytometry, and microscopic methods. This study willgenerate a library of protistan images and ribosomal DNA sequences which will contribute noveltaxonomic and ecological data to the scientific community and publicly accessible databases.The taxonomic survey will be supplemented by field experiments, which will examine the effectof differences in microbial community composition on two functional aspects of the community:growth and grazing.Protists (unicellular eukaryotic microorganisms) play an important role in aquatic ecosystemsby regulating bacterial and primary production and regenerating the bulk of inorganic nutrientsbut the relationship between their diversity and functions remains poorly understood. This studywill increase our knowledge of microbial life in critical estuarine/coastal wetland environmentsand improve our ability to predict complex and dynamic microbial processes in the Great Lakesand the coastal ocean. On a yet broader scale, the study will provide new insights about therelationship between biodiversity and function in natural communities. Finally, this study willhave an impact on the infrastructure of education within the PIs institutions and beyond.Students at UA and FIU involved in the project will experience a wide range of professionalactivities and will be involved in all aspects of the research enterprise. In addition, this projectwill provide information for the OWC NERR visitor center exhibit and thus contribute to thegeneral public understanding of the importance of preserving biodiversity and estuarine/wetlandenvironments. The results of this study will be invaluable in informing decisions on conservationand environmental management these important habitats that have become increasing threatened.

阿克伦大学的Peter Lavrentyev和Robert Duff博士获得了一笔赠款，用于研究NOAA老妇溪国家河口研究保护区（OWC NERR）中与其关键功能有关的浮游原生生物的分类组成。样本将收集从四个站沿着河流，伊利湖样带每月一次，结合正在进行的长期监测。微生物群落结构，分布和动态将在不同的水文和气候条件下使用分子，数字视频，流式细胞术和显微镜方法进行检查。这项研究将产生一个原生生物图像和核糖体DNA序列库，为科学界和公众可访问的数据库提供新的分类和生态数据。分类调查将由实地实验补充，实地实验将研究微生物群落组成的差异对群落两个功能方面的影响：生长和放牧。原生生物（单细胞真核微生物）通过调节细菌和初级生产以及再生大量无机营养物，其多样性与功能之间的关系仍然知之甚少。这项研究将增加我们对关键河口/沿海湿地环境中微生物生命的认识，提高我们预测大湖和沿海海洋中复杂和动态微生物过程的能力。在更广泛的范围内，这项研究将为自然群落中生物多样性和功能之间的关系提供新的见解。最后，这项研究将对PI机构内外的教育基础设施产生影响。参与该项目的UA和FIU学生将体验广泛的专业活动，并将参与研究企业的各个方面。此外，该项目还为OWC NERR游客中心展览提供信息，从而有助于公众了解保护生物多样性和河口/湿地生态系统的重要性。这项研究的结果将是非常宝贵的，为保护和环境管理这些日益受到威胁的重要栖息地的决定提供信息。