NER: Investigation of Molecular Stochastic Motion in a Nanofluidic Channel

NER：纳流体通道中分子随机运动的研究

• 批准号: 0304106
• 负责人: Jongyoon Han
• 金额: $ 10万
• 依托单位: Massachusetts Institute of Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-15 至 2005-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0304106&HistoricalAwards=false
• 关键词: NER; Investigation; Molecular; Stochastic; Motion

ABSTRACTCTS-0304106Jongyoon Han, MITStochastic motion of biomolecules within a confined space will be studied, using regular nanofluidic channels made by standard MEMS technology. Nanofluidic channels with known shape and depth (10~1000nm) will be designed and fabricated on Si or polymer substrates to confine molecular motion at least in one dimension, and the stochastic motion of single molecules will be followed by the fluorescence correlation spectroscopy technique. The changes in molecular stochastic motion, caused either by the confinement effect or the interaction with Debye layer, will be studied. The result of this research could provide insights in understanding biomolecular transport in the confined intracellular spaces. Also, it could generate new ideas for controlling molecules within nanostructures, which has a direct application in biomolecule sensing and separation. Also, the concept of molecular manipulation by nanofluidic channels could have broad impacts and applications in various science and engineering areas.The project is being funded by three programs in CTS:Particulate and Multiphase Processes Thermal Transport and Thermal Processing Separation and Purification Processes

摘要 CTS-0304106Jongyoon Han，麻省理工学院将使用标准 MEMS 技术制造的规则纳米流体通道来研究有限空间内生物分子的随机运动。 在硅或聚合物基底上设计和制造已知形状和深度（10~1000nm）的纳流通道，将分子运动限制在至少一维上，并通过荧光相关光谱技术跟踪单分子的随机运动。将研究由限制效应或与德拜层的相互作用引起的分子随机运动的变化。 这项研究的结果可以为理解有限的细胞内空间中的生物分子运输提供见解。此外，它还可以产生控制纳米结构内分子的新想法，这在生物分子传感和分离方面有直接应用。此外，通过纳米流体通道进行分子操纵的概念可能会在各个科学和工程领域产生广泛的影响和应用。该项目由 CTS 的三个项目资助：颗粒和多相过程热传输和热处理分离和纯化过程