Molecular Updates for Chicken and Zebrafish Embryology Laboratory Exercises

鸡和斑马鱼胚胎学实验室练习的分子更新

• 批准号: 0310887
• 负责人: Alyssa Perz-Edwards
• 金额: --
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-15 至 2008-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0310887&HistoricalAwards=false
• 关键词: Molecular; Updates; Chicken; Zebrafish; Embryology

Undergraduate labs in developmental biology are usually limited to experiments in classical embryology, and the experimental offerings do not include advances made using modern molecular and genetic tools. This project is adapting and implementing methods from the research literature into the undergraduate classroom in two modules. The first module is adapting electroporation technology for chicken embryos, so students can introduce DNA constructs containing molecules that can specify limb type or induce ectopic wing formation. The second module involves introducing morpholinos into zebrafish embryos to reduce translation of specific molecules that affect heart development. The proposed changes update traditional laboratories offered in the Duke course, Experiments in Development and Molecular Genetics, by introducing molecular technology. Because the chickens and zebrafish are widely available and easy to culture, these exercises may have broad appeal for instructors interested in adding molecular technology to developmental biology laboratories. Therefore, descriptions of the exercises are being submitted to educational journals for publication, made available on the web and publicized at meetings. The broader impacts of the project include: the enhancement of faculty development and the integration of research and technology in undergraduate education.

发育生物学的本科实验室通常仅限于经典胚胎学实验，并且实验产品不包括使用现代分子和遗传工具取得的进展。该项目分两个模块将研究文献中的方法应用到本科生课堂中。第一个模块是对鸡胚胎采用电穿孔技术，因此学生可以引入含有可以指定肢体类型或诱导异位翅膀形成的分子的 DNA 构建体。第二个模块涉及将吗啉引入斑马鱼胚胎中，以减少影响心脏发育的特定分子的翻译。拟议的改变通过引入分子技术更新了杜克大学发育和分子遗传学实验课程中提供的传统实验室。由于鸡和斑马鱼广泛存在且易于培养，因此这些练习可能对有兴趣在发育生物学实验室中添加分子技术的教师具有广泛的吸引力。因此，练习的描述将提交给教育期刊以供出版、在网络上提供并在会议上公布。该项目更广泛的影响包括：加强教师发展以及本科教育中研究与技术的整合。