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Early Molecular Events in Plant-Fungus Interactions

植物-真菌相互作用的早期分子事件

基本信息

批准号：
0312183
负责人：
P. Kolattukudy
金额：
$ 4.64万
依托单位：
The University of Central Florida Board of Trustees
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2002
资助国家：
美国
起止时间：
2002-12-31 至 2003-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0312183&HistoricalAwards=false
关键词：
Early Molecular Events Plant Fungus

项目摘要

9816868KolattukudyThe overall objective of this research program is to elucidate the early molecular events involved in the interactions of fungi with the plant cuticle using Colletotrichum gloeosporioides and Nectria haematococca as test organisms. These interactions include the first priming stage that involves the contact of the fungal conidia with hard surface followed by germination and appressorium formation triggered by the host's chemical signals, and penetration of the cuticle and pectin barriers of the host. Early molecular events involved in the interaction of C. gloeosporioides with its host will be elucidated by cloning the genes induced by contact of the conidia with hard host surface. The Colletotrichum Hard surface Induced Protein (chip) genes will be disrupted to assess whether they are required for pathogenesis and the functions of the chip gene products will be explored. How hydroxyfatty acids of plants activate cutinase gene transcription in N. haematococca will be elucidated by cloning the transcription factors involved in the activation and by assessing their functions in yeast transformed with marker genes and the cloned transacting factors. The role of pectate lyase in pathogenesis will be tested by assessing the virulence of single and double disruptants in which pectin-inducible pelA gene and/or host-inducible pelD gene are disrupted. The host factor that is responsible for the in Planta expression of pelD gene will be purified and identified. The results of the proposed studies will provide new insights into the role of the cuticle in pathogenesis, and may help to devise new methods to control fungal diseases of plants that constitute one of the major causes of losses in food and fiber production.

9816868 Kolattukudy本研究计划的总体目标是阐明真菌与植物角质层相互作用的早期分子事件，使用Colletotrichum gloeosporioides和Nectria haematococca作为测试生物。这些相互作用包括第一引发阶段，其涉及真菌分生孢子与硬表面的接触，随后由宿主的化学信号触发萌发和附着胞形成，以及穿透宿主的角质层和果胶屏障。 C.通过克隆分生孢子与硬寄主表面接触诱导的相关基因，阐明胶孢霉与寄主的关系。将破坏刺盘孢菌硬表面诱导蛋白（芯片）基因，以评估它们是否是发病机制所需的，并将探索芯片基因产物的功能。植物羟基脂肪酸如何激活N.将通过克隆参与激活的转录因子和通过评估它们在用标记基因和克隆的反式作用因子转化的酵母中的功能来阐明红球菌属。果胶酸裂解酶在发病机理中的作用将通过评估其中果胶诱导型pelA基因和/或宿主诱导型pelD基因被破坏的单和双破坏剂的毒力来测试。将负责pelD基因在植物中表达的宿主因子纯化并鉴定。拟议研究的结果将为角质层在发病机理中的作用提供新的见解，并可能有助于设计新的方法来控制植物真菌疾病，这些疾病是造成粮食和纤维生产损失的主要原因之一。