Vanadium Complex Interaction with Lipids and Transport across Lipid Membranes

科罗拉多州立大学化学系的Debbie C.Crans和Nancy E.Levinger得到了无机化学、生物无机化学和有机金属化学计划的支持，用于探索金属络合物与脂质相互作用的基础研究。他们将合成钒(IV)和钒(V)与吡啶甲酸酯、二吡啶甲酸酯和乙酰丙酮等配体的络合物。他们将在分散的脂类、反胶束、囊泡和脂质体存在的情况下，研究它们在水溶液中的性质。表征手段包括多核核磁共振、电子顺磁共振、紫外可见光谱、红外光谱和拉曼光谱。脉冲场梯度核磁共振和时间分辨各向异性也将测量金属络合物与脂质组件的相互作用。通过表面二次谐波的产生来测量金属络合物在脂类双层中的潜在传输。金属离子参与许多代谢过程。钒络合物已被证明可以使糖尿病患者的高血糖水平正常化。该项目将深入了解金属络合物相互作用和跨双分子层运输的基本方面，这对于设计治疗糖尿病和其他代谢紊乱的新疗法至关重要。该项目将吸引本科生和研究生参与，并利用各种最先进的光谱工具提高女性在生物无机和生物物理研究中的能见度。

Debbie C. Crans and Nancy E. Levinger, Department of Chemistry, Colorado State University, are supported by the Inorganic, Bioinorganic and Organometallic Chemistry Program for fundamental studies exploring how metal complexes interact with lipids. They will synthesize vanadium(IV) and vanadium(V) complexes with ligands such as picolinate, dipicolinate, and acetylacetonate. They will study their properties in aqueous solution, in the presence of dispersed lipids, reverse micelles, vesicles and liposomes. The tools for characterization of the systems include multinuclear NMR, EPR, UV/visible, IR and Raman spectroscopy. Pulsed field gradient NMR and time-resolved anisotropy will also measure interactions of the metal complexes with the lipid assemblies. The potential transport of the metal complexes across lipid bilayers will be measured using surface second harmonic generation. Metal ions are involved in many metabolic processes. Vanadium complexes have been shown to normalize elevated glucose levels in diabetic humans. This project will provide insight into the fundamental aspects of metal complex interactions and transport across bilayers that are critical for the design of new therapeutics for diabetes and other metabolic disorders. The project will engage students, both undergraduate and graduate, and enhance the visibility of women in bioinorganic and biophysical research using a variety of state-of-the-art spectroscopic tools.