Role of Steroid Sulfonation as a Mechanism Controlling the Flow of Substrates through the Cascade of Steroidogenic Enzymes in the Porcine Testis

类固醇磺化作为通过猪睾丸中类固醇生成酶级联控制底物流动的机制的作用

• 批准号: 163148736
• 负责人: Professor Dr. Gerhard Schuler
• 金额: --
• 依托单位: Tierklinik für Reproduktionsmedizin und Neugeborenenkunde
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/163148736?language=en
• 关键词: Role; Steroid; Sulfonation; Mechanism; Controlling

Whereas in males of many mammalian species estrogens circulate in peripheral blood predominantly as free forms at low concentrations, in the boar large quantities of free and conjugated estrogens (with estrone sulfate being the predominant estrogen) occur in peripheral blood. In the porcine testicular-epididymal compartment estrogen receptors, steroid sulfatase (StS), estrogen sulfotransferase, aromatase and at least one putative steroid sulfate transporter (SOAT) are expressed in close proximity with each other, pointing to the synthesis, hydrolysis and transport of sulfated estrogens as local controlling elements of estrogenic actions in addition to de novo synthesis of free estrogens. The final goal of this project is to study in vivo the effect of StS inhibition on gonadal steroidogenesis and spermatogenesis in boars. Thus the aims of the first period of the project are 1) to close still existing gaps in the knowledge on the expression patterns of StS and the estrogen specific sulfotransferase SULT1E1 in the porcine testicular-epididymal compartment 2) to analyze the cellular localizations of SOAT and other putative steroid sulfate carriers such as OSCP1 in the testicular– epididymal compartment of the boar in relation to age, steroidogenesis and spermatogenesis and 3) to identify an effective StS inhibitor for the intended in vivo application in the boar.

在许多哺乳动物的雄性中，雌激素主要以低浓度的游离形式在外周血中循环，而在公猪中，外周血中存在大量的游离雌激素和结合雌激素（硫酸雌酮是主要的雌激素）。在猪睾丸-附睾区雌激素受体中，类固醇硫酸酯酶（StS）、雌激素磺基转移酶、芳香酶和至少一种推定的类固醇硫酸盐转运蛋白（SOAT）彼此紧密表达，表明硫酸化雌激素的合成、水解和转运除了从头开始外，也是雌激素作用的局部控制元件。 游离雌激素的合成。该项目的最终目标是在体内研究 StS 抑制对公猪性腺类固醇生成和精子发生的影响。因此，该项目第一阶段的目标是 1) 弥合猪睾丸-附睾区室中 StS 和雌激素特异性磺基转移酶 SULT1E1 表达模式方面仍然存在的知识空白 2) 分析公猪睾丸-附睾区室中 SOAT 和其他假定的类固醇硫酸盐载体（例如 OSCP1）的细胞定位 年龄、类固醇生成和精子生成；3) 确定一种有效的 StS 抑制剂，用于公猪体内的预期应用。

项目成果

Free and sulfated steroids secretion in postpubertal boars (Sus scrofa domestica).

青春期后公猪（Sus scrofa Domestica）的游离和硫酸化类固醇分泌

• DOI: 10.1530/rep-14-0193
• 发表时间: 2014
• 期刊: Reproduction
• 影响因子: 3.8
• 作者: Schuler G;Dezhkam Y;Bingsohn L;Hoffmann B;Failing K;Galuska CE;Hartmann MF;Sánchez- Guijo A;Wudy SA
• 通讯作者: Wudy SA