Essential (and Dispensable) Genes in Escherichia Coli

大肠杆菌中必需的（和非必需的）基因

• 批准号: 0315788
• 负责人: William Reznikoff
• 金额: $ 44.77万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-15 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0315788&HistoricalAwards=false
• 关键词: Essential; Dispensable; Genes; Escherichia; Coli

This project utilizes Tn5 derived DNA transposition systems to address several fundamental questions in bacterial genomics. The experiments will define the compliment of essential, dispensable and conditionally essential genes in the Escherichia coli K12 genome. This effort will provide a powerful tool for studying the function of genes of unknown function. In addition the E. coli K12 gene content will be reduced to the minimal necessary for survival for aerobic growth in rich medium. This will result in the generation of an organism that is more amenable to metabolic modeling. Finally, the techniques will be used as a tool for studying in vivo chromosome condensation. The project has broad impacts beyond basic science discoveries. The methodologies to be employed can be used to study any bacterial species amenable to DNA electroporation technology and thus can be used, for instance to identify functions necessary for pathogen survival in host organisms. In addition, previous work on this technology has resulted in the generation of a number of patents that have been licensed to a biotechnology supply company. These types of practical discoveries will continue. Finally, the research program has always been used as a vehicle for undergraduate education especially in regards to underrepresented minorities. This has been accomplished by having undergraduates as active participants in the research projects.

该项目利用 Tn5 衍生的 DNA 转座系统来解决细菌基因组学中的几个基本问​​题。这些实验将定义大肠杆菌 K12 基因组中必需、可有可无和条件必需基因的补充。这项工作将为研究未知功能基因的功能提供强大的工具。此外，大肠杆菌 K12 基因含量将减少至在丰富培养基中有氧生长生存所需的最低限度。这将导致产生更适合代谢模型的生物体。最后，该技术将用作研究体内染色体凝聚的工具。该项目具有超出基础科学发现的广泛影响。所采用的方法可用于研究任何适合DNA电穿孔技术的细菌物种，因此可用于例如鉴定病原体在宿主生物体中生存所必需的功能。此外，之前对该技术的研究已经产生了许多专利，这些专利已授权给一家生物技术供应公司。这些类型的实际发现将会继续下去。最后，该研究项目一直被用作本科教育的工具，特别是对于代表性不足的少数族裔而言。这是通过让本科生积极参与研究项目来实现的。