权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Conformational dynamics and regulation of bacterial type I maltose ABC transporters

细菌 I 型麦芽糖 ABC 转运蛋白的构象动力学和调控

基本信息

批准号：
163317791
负责人：
Professorin Dr. Enrica Bordignon
金额：
--
依托单位：
Arbeitsgruppe Physiologie von Mikroorganismen
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2010
资助国家：
德国
起止时间：
2009-12-31 至 2017-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/163317791?language=en
关键词：
Conformational dynamics regulation bacterial type

项目摘要

ATP-binding cassette (ABC) transporters are membrane proteins that mediate the uptake or export of an enormous variety of substrates at the expense of ATP. ABC transporters share a common structural organization comprising two transmembrane domains that form the translocation pore and two nucleotide-binding domains that hydrolyse ATP. The maltose transporter of E. coli/Salmonella has served as the model for canonical ABC import systems which require an additional component, a substrate-specific binding protein for function. Based on a plethora of genetic, biochemical and structural data we propose to investigate the dynamics of the system in detail. To this end, we will combine site-specific chemical crosslinking with electron paramagnetic resonance spectroscopy to identify individual steps of the transport cycle. The results, together with molecular modelling will eventually lead to a complete picture of the transporter’s motional changes and thus to the understanding of the transport mechanism at the molecular level. Furthermore, we will address by the same techniques completed by fluorescence-based technology (FP-FRET, FCS), the physiologically relevant question of the mechanism by which the glucose transporter component, EIIAGlc, inhibits the maltose transporter in a process termed ‘inducer exclusion’.

ATP结合盒（ABC）转运蛋白是一种以ATP为代价介导多种底物摄取或输出的膜蛋白。ABC转运蛋白共有一个共同的结构组织，包括两个形成易位孔的跨膜结构域和两个水解ATP的核苷酸结合结构域。E.大肠杆菌/沙门氏菌已作为典型ABC输入系统的模型，该系统需要额外的组分，即用于功能的底物特异性结合蛋白。基于大量的遗传，生化和结构数据，我们建议详细研究系统的动力学。为此，我们将结合联合收割机网站特定的化学交联与电子顺磁共振光谱，以确定个别步骤的运输周期。结果，连同分子建模将最终导致一个完整的图片的运输的运动变化，从而在分子水平上的运输机制的理解。此外，我们将通过基于荧光的技术（FP-FRET，FCS）完成的相同技术来解决葡萄糖转运蛋白组分EIIAGlc在称为“诱导物排除”的过程中抑制麦芽糖转运蛋白的机制的生理相关问题。