SBIR Phase I: Low Volume Reloadable Printhead for Microarray Production

SBIR 第一阶段：用于微阵列生产的小容量可重装打印头

• 批准号: 0318870
• 负责人: Bogdan Antohe
• 金额: $ 10万
• 依托单位: MicroFab Technologies Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2003-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0318870&HistoricalAwards=false
• 关键词: SBIR; Phase; Low; Volume; Reloadable

This Small Business Innovation Research (SBIR) Phase I project will design and build a reloadable printhead for ink-jet deposition of biological fluids into microarrays. Production of mid range microarrays (hundreds of different fluids) is not best addressed by the existing tools. Photolithography is expensive and with long turnaround time and existing single jet devices have large volumes. The reloadable printhead will fill this market segment. The first step in this project will be to optimize the geometrical dimensions of the printhead using numerical simulations and models for a low loading volume and for best operation. Special printheads will be built for evaluation and simulation verification. The second step would consist of the actual fabrication of the devices. Functionality of the fabricated printheads will be verified using a set of oligonucleotide probes designed to detect polymorphism in the Human Leukocyte Antigen (HLA) genes. Current research indicates a correlation between the class II HLA polymorphism and the occurrence of cervical cancer. Microarrays produced with a reduced set of probes will be fabricated and hybridized with prefabricated target DNA. A design of a full probe set will be made considering the use of the reloadable printhead to fabricate microarrays for investigating genetic susceptibility of cervical cancer.The commercial application of this project is in the area of microarrays. On completion of the work through Phase I and Phase II, revenues are expected to be obtained through sales of the printhead and printing systems for microarray production, and through actual production .

这个小企业创新研究（SBIR）第一阶段项目将设计和建造一个可重新加载的打印头，用于喷墨沉积生物流体到微阵列中。生产中程微阵列（数百种不同的流体）不是最好的解决现有的工具。光刻技术成本高，周转时间长，现有的单喷设备体积大。可重新装填的打印头将填补这一细分市场。该项目的第一步将是利用数值模拟和模型优化打印头的几何尺寸，以实现低负载体积和最佳操作。将建造专门的打印头进行评估和仿真验证。第二步将包括设备的实际制造。将使用一组设计用于检测人类白细胞抗原（HLA）基因多态性的寡核苷酸探针来验证制备的打印头的功能。目前的研究表明，II类HLA多态性与宫颈癌的发生存在相关性。用减少的探针制作的微阵列将与预制的目标DNA杂交。我们将设计一套完整的探针，考虑使用可重新加载的打印头来制造微阵列，以研究宫颈癌的遗传易感性。该项目的商业应用是在微阵列领域。在第一阶段和第二阶段完成工作后，预计将通过销售用于微阵列生产的打印头和打印系统以及通过实际生产获得收入。