Modification of the ABC Program and the Evolution of Floral Novelty

ABC程序的修改与花艺新奇的演变

• 批准号: 0319103
• 负责人: Elena Kramer
• 金额: $ 33.9万
• 依托单位: Harvard University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0319103&HistoricalAwards=false
• 关键词: Modification; ABC; Program; Evolution; Floral

Kramer0319103Understanding how new morphological characters evolve has become a major focus of the rapidly growing field of comparative developmental genetics. In flowering plants, it has been proposed that radical changes in floral morphology may have been mediated by simple shifts in the expression domains of homeotic floral organ identity genes. This hypothesis has not been carefully tested, however, and still leaves unexplained the question of how the underlying program may have been modified in cases where a simple "sliding boundary" model is insufficient to explain morphology. These issues can be addressed through studies of the lower eudicot family Ranunculaceae, which contains a great diversity of floral morphology. Although preliminary data suggest that the ABC program in this family has been reshaped by gene duplications, further characterization of ABC gene homologs is necessary to clarify these findings and test their broader applicability. To these ends, the objectives of the project are: 1) to analyze the expression patterns of AP3, PI, AG, and AP1 homologs in Aquilegia vulgaris using in situ hybridization; 2) to characterize homeotic mutants of A. vulgaris using SEM, gene expression studies, candidate gene sequencing and analysis of genetic segregation; 3) to determine the protein dimerization and DNA binding capabilities of AP3 and PI homologs from Aquilegia vulgaris; and 4) to use antibody localization to determine the protein distribution of AP3, PI, AG and AP1 gene products in Aquilegia vulgaris, Xanthorhiza simplicissima, Clematis integrifolia, Clematis alpina and Ranunculus ficaria. In addition to investigating the evolution of floral morphology, these studies will address issues such as the conservation of organ identity gene function outside the core eudicots and the role of gene duplication in the evolution of novel developmental programs. In terms of broader impact, this project will provide training for undergraduate, graduate and postdoctoral students.

Kramer 0319103了解新的形态特征是如何进化的已经成为快速发展的比较发育遗传学领域的一个主要焦点。 在显花植物中，有人提出花形态的根本变化可能是由同源异型花器官身份基因的表达结构域的简单变化介导的。 然而，这一假设尚未得到仔细的检验，并且仍然没有解释在简单的“滑动边界”模型不足以解释形态学的情况下，底层程序如何被修改的问题。 这些问题可以通过对包含着丰富的花形态多样性的低等真双子叶植物毛茛科的研究来解决。 虽然初步的数据表明，在这个家庭的ABC程序已被重塑的基因复制，ABC基因同源物的进一步表征是必要的，以澄清这些发现，并测试其更广泛的适用性。 为此，本研究的目的是：1）利用原位杂交技术分析Aquilegia vulgaris中AP 3、PI、AG和AP 1同源基因的表达模式;通过扫描电镜、基因表达研究、候选基因测序和遗传分离分析等方法，对Aquilegia vulgaris的AP 3和PI同源物进行了蛋白质二聚化和DNA结合能力的研究; 4）利用抗体定位技术，对Aquilegia vulgaris、Xanthorhiza simplicissima、威灵仙高山铁线莲和毛茛。 除了调查花形态的进化，这些研究将解决的问题，如核心真双子叶植物外的器官身份基因功能的保护和基因复制在新的发育程序的进化中的作用。 就更广泛的影响而言，该项目将为本科生、研究生和博士后学生提供培训。