SBIR Phase I: Targeting Low Abundance Proteomics: Selective Enrichment in Combined Displacement Chromatography and Isotachophoresis

该小企业创新研究（SBIR）一期项目拟开发并演示一个多维蛋白质/肽分离/浓缩平台，该平台能够通过“平衡”或“平衡”蛋白质补体的浓度来降低相对蛋白质丰度的范围。这种对低丰度蛋白质的选择性增强可以大大减少复杂样品中相对蛋白质丰度的范围，并显着提高常规质谱法在使用小细胞群或有限组织样品进行全局蛋白质组学研究时的动态范围和灵敏度。该项目的商业应用是在蛋白质组学领域。所提出的生物分析方法的发展预计将对生物制药公司的筛选/药物发现工作有价值。

This Small Business Innovation Research (SBIR) Phase I project proposes to develop and demonstrate a multidimensional protein/peptide separation/concentration platform, capable of reducing the range of relative protein abundances by "balancing" or "leveling" the concentrations of the protein complement. Such selective enhancement toward low abundance proteins can drastically reduce the range of relative protein abundances in complex samples and significantly enhance the dynamic range and sensitivity of conventional mass spectrometry toward global proteomic studies using small cell populations or limited tissue samples.The commercial application of this project is in the area of proteomics. The development of the proposed bioanalytical methodologies are expected to be valuable in screening / drug discovery efforts by bio-pharmaceutical companies.