SBIR Phase I: Targeting Low Abundance Proteomics: Selective Enrichment in Combined Displacement Chromatography and Isotachophoresis

SBIR 第一阶段：针对低丰度蛋白质组学：组合置换色谱和等速电泳的选择性富集

• 批准号: 0319722
• 负责人: Jun Gao
• 金额: $ 10万
• 依托单位: CALIBRANT BIOSYSTEMS INC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0319722&HistoricalAwards=false
• 关键词: SBIR; Phase; Targeting; Low; Abundance

This Small Business Innovation Research (SBIR) Phase I project proposes to develop and demonstrate a multidimensional protein/peptide separation/concentration platform, capable of reducing the range of relative protein abundances by "balancing" or "leveling" the concentrations of the protein complement. Such selective enhancement toward low abundance proteins can drastically reduce the range of relative protein abundances in complex samples and significantly enhance the dynamic range and sensitivity of conventional mass spectrometry toward global proteomic studies using small cell populations or limited tissue samples.The commercial application of this project is in the area of proteomics. The development of the proposed bioanalytical methodologies are expected to be valuable in screening / drug discovery efforts by bio-pharmaceutical companies.

这个小型企业创新研究（SBIR）第一阶段项目提出开发和展示一个多维蛋白质/肽分离/浓缩平台，能够通过“平衡”或“平衡”蛋白质补体的浓度来减少相对蛋白质丰度的范围。这种对低丰度蛋白质的选择性增强可以显著降低复杂样品中蛋白质相对丰度的范围，并显著提高常规质谱法的动态范围和灵敏度，用于小细胞群体或有限组织样品的全球蛋白质组学研究。拟议的生物分析方法的发展，预计将是有价值的筛选/药物发现的努力，生物制药公司。