SBIR Phase I: Use of Histone-Like Protein to Assess the Health of Rainbow Trout

SBIR 第一阶段：使用组蛋白样蛋白评估虹鳟鱼的健康状况

• 批准号: 0320324
• 负责人: Paul Borron
• 金额: $ 9.62万
• 依托单位: Norcarex Bio Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0320324&HistoricalAwards=false
• 关键词: SBIR; Phase; Use; Histone; Like

This Small Business Innovation Research (SBIR) Phase I project proposes to explore the novel use of an immune protein for measuring stress in rainbow trout. No convenient tests are currently available to measure stress, a condition that often leads to disease. Accurate stress measurements will dramatically reduce disease losses, the major cause of economic losses in salmonid aquaculture. HLP-1 is a potent antibiotic immune protein that is naturally present in many tissues of rainbow trout. This protein is expected to play a critical role in protecting trout against disease. Stress has been noted to cause a dramatic decrease in HLP-1 levels. This Phase I Project will explore the feasibility of measuring HLP-1 in gill tissue using a simple, antibody-based methodology (ELISA). This assay should have sufficient sensitivity and specificity to be used for commercial development of a low- cost, field-deployable, immunoassay prototype test.The commercial application of this project is in the field of aquaculture. The proposed technology will find use in aquacultured food fish, as well as for health monitoring in pet fish, laboratory animals and wild fish populations.

这个小型企业创新研究 (SBIR) 第一阶段项目提议探索免疫蛋白测量虹鳟鱼压力的新用途。目前还没有方便的测试来测量压力，这种情况通常会导致疾病。准确的应激测量将大大减少疾病损失，这是鲑科鱼水产养殖经济损失的主要原因。 HLP-1 是一种强效抗生素免疫蛋白，天然存在于虹鳟鱼的许多组织中。这种蛋白质有望在保护鳟鱼免受疾病侵害方面发挥关键作用。研究发现，压力会导致 HLP-1 水平急剧下降。该第一阶段项目将探索使用简单的基于抗体的方法 (ELISA) 测量鳃组织中 HLP-1 的可行性。该测定应具有足够的灵敏度和特异性，以用于低成本、可现场部署的免疫测定原型测试的商业开发。该项目的商业应用是在水产养殖领域。拟议的技术将用于水产养殖食用鱼，以及宠物鱼、实验动物和野生鱼类种群的健康监测。